A chemical dosimeter for the determination of the photodynamic activity of photosensitizers
A chemical dosimeter for the determination of the photodynamic activity of photosensitizers
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DOI：
10.1016/s0009-8981(98)00045-x
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发表时间：
1998-06-08
影响因子：
5
通讯作者：
Schlag, PM
中科院分区：
文献类型：
--
作者：
Fischer, F;Graschew, G;Schlag, PM
尿酸是一种已知的单线态氧清除剂，在生理水溶液中作为化学剂量计用于光动力治疗。尿酸试验采用激光照射含有UA和光敏剂的溶液后，在293 nm处尿酸（UA）吸光度的下降，作为光敏剂相对光动力活性的快速评价。制定了尿酸试验标准程序。为了比较不同光敏剂或不同辐照条件下的光动力活性，提出了一种基于尿酸试验的光动力活性量表。通过比较光敏剂Photofrin II与其他两种光敏剂(5,10,15,20-tetrakis-[4-hydroxyphenyl]- 21h,23H-porphyrin （TOP）和5,10,15,20-tetrakis-[4-carboxyphenyl]- 21h,23H-porphyrin （TCPP)，两者均由甲氧基聚乙二醇衍生（TOP- mpv和TCPP- amp）作为大分子载体）的辐照波长和光敏剂浓度的UA吸光度下降的变化，给出了尿酸试验操作的例子。用所提出的光动力活性标度给出了三种光敏剂的光动力活性。(C) 1998 Elsevier Science B.V.版权所有
Uric acid a known singlet oxygen scavenger, was investigated as a chemical dosimeter in physiological aqueous solution for use in photodynamic therapy. The uric acid test takes the decrease in uric acid (UA) absorbance at 293 nm after laser light irradiation of a solution containing UA and a photosensitizer as a rapid evaluation of relative photodynamic activities of the photosensitizer. A uric acid test standard procedure was defined. To compare photodynamic activity of different photosensitizers or irradiation conditions a proposal for a photodynamic activity scale based on the uric acid test is given. Examples of uric acid test operation are given by comparing the changes in UA absorbance decrease with respect to irradiation wavelength and to photosensitizer concentration of Photofrin II with that of two other photosensitizers (5,10,15,20-tetrakis-[4-hydroxyphenyl]-21H,23H-porphyrin (TOP) and 5,10,15,20-tetrakis-[4-carboxyphenyl)-21H, 23H-porphyrin (TCPP), both derivatized with methoxypolyethyleneglycol (TOP-MPV and TCPP-AMP) as a macromolecular carrier). The photodynamic activity of the three photosensitizers using the proposed photodynamic activity scale is given. (C) 1998 Elsevier Science B.V. All rights reserved.