Diversities of coral-associated bacteria differ with location, but not species, for three acroporid corals on the Great Barrier Reef
Diversities of coral-associated bacteria differ with location, but not species, for three acroporid corals on the Great Barrier Reef
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DOI：
10.1111/j.1574-6941.2009.00666.x
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Bourne, David G.
中科院分区：
文献类型：
--
作者：
Littman, Raechel A.;Willis, Bette L.;Bourne, David G.
在大堡礁的两个地点（磁岛和俄耳甫斯岛）与三种鹿角珊瑚（鹿角珊瑚millepora，鹿角珊瑚tenuis和鹿角珊瑚valida）的细菌群落的多样性模式进行了比较，以更好地了解珊瑚微生物共生的性质和特异性。三种独立于培养物的技术证明了每个珊瑚物种的重复样本中细菌群落的一致性，证实了珊瑚与特定的微生物群有关。配置文件也保守的所有三个物种的鹿角珊瑚在每个位置，这表明密切相关的珊瑚相同属港口类似的细菌类型。A.细菌群落特征在俄耳甫斯岛millepora是一致的，在全年收集的样品，表明一个稳定的社区，尽管时间的变化。然而，DGGE和T-RFLP配置文件不同的珊瑚礁。非度量多维尺度的T-RFLP配置文件显示，样本分组根据位置，而不是珊瑚物种。虽然相似的序列检索到的克隆库的珊瑚在磁和俄耳甫斯岛，在相对优势的细菌核糖体型的差异内的图书馆驱动细菌群落结构在不同的地理位置。这些结果表明，某些细菌群与珊瑚特别相关，但占主导地位的细菌属不同的地理间隔的珊瑚。
Patterns in the diversity of bacterial communities associated with three species of Acropora (Acropora millepora, Acropora tenuis and Acropora valida) were compared at two locations (Magnetic Island and Orpheus Island) on the Great Barrier Reef to better understand the nature and specificity of coral-microbial symbioses. Three culture-independent techniques demonstrated consistent bacterial communities among replicate samples of each coral species, confirming that corals associate with specific microbiota. Profiles were also conserved among all three species of Acropora within each location, suggesting that closely related corals of the same genus harbor similar bacterial types. Bacterial community profiles of A. millepora at Orpheus Island were consistent in samples collected throughout the year, indicating a stable community despite temporal changes. However, DGGE and T-RFLP profiles differed on corals from different reefs. Nonmetric multidimensional scaling of T-RFLP profiles showed that samples grouped according to location rather than coral species. Although similar sequences were retrieved from clone libraries of corals at both Magnetic and Orpheus Island, differences in the relative dominant bacterial ribotypes within the libraries drive bacterial community structure at different geographical locations. These results indicate certain bacterial groups associated specifically with corals, but the dominant bacterial genera differ between geographically-spaced corals.