De novo transcriptome assembly of the Southern Ocean copepod Rhincalanus gigas sheds light on developmental changes in gene expression
De novo transcriptome assembly of the Southern Ocean copepod Rhincalanus gigas sheds light on developmental changes in gene expression
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DOI：
10.1016/j.margen.2021.100835
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发表时间：
2021-07-01
期刊：
影响因子：
1.9
通讯作者：
Tarrant, Ann M.
中科院分区：
文献类型：
--
作者：
Berger, Cory A.;Steinberg, Deborah K.;Tarrant, Ann M.
桡足类是小型甲壳类动物，在丰度和生物量方面占大多数浮游动物群落的主导地位。在极地海洋中，一个大型的储存脂质的桡足类的子集占据食物网的中心位置，因为它们在连接浮游植物和微型浮游动物与更高的营养级的重要作用。在本文中，我们产生了一个高品质的从头转录Rhincalanus gigas，最大的，其中最丰富的南大洋桡足类。然后，我们进行了转录分析，以表征后期青少年和成年女性之间的发展过渡。我们发现那个少年R. Gigas相对于雌性显著上调脂质合成和糖酵解途径，作为发育基因表达程序的一部分，其还涉及诸如肌肉生长、几丁质形成和离子转运的过程。这项研究提供了第一个内Rhincalanus gigas或任何特有的南大洋桡足类的发育转变的转录谱，从而扩展了我们对桡足类分子生理学的理解。
Copepods are small crustaceans that dominate most zooplankton communities in terms of both abundance and biomass. In the polar oceans, a subset of large lipid-storing copepods occupy central positions in the food web because of their important role in linking phytoplankton and microzooplankton with higher trophic levels. In this paper, we generated a high-quality de novo transcriptome for Rhincalanus gigas, the largest-and among the most abundant-of the Southern Ocean copepods. We then conducted transcriptional profiling to characterize the developmental transition between late-stage juveniles and adult females. We found that juvenile R. gigas substantially upregulate lipid synthesis and glycolysis pathways relative to females, as part of a developmental gene expression program that also implicates processes such as muscle growth, chitin formation, and ion transport. This study provides the first transcriptional profile of a developmental transition within Rhincalanus gigas or any endemic Southern Ocean copepod, thereby extending our understanding of copepod molecular physiology.