Human galectin-1,-2, and-4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells
Human galectin-1,-2, and-4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells
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DOI：
10.1182/blood-2006-03-007153
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发表时间：
2007-01-01
期刊：
影响因子：
20.3
通讯作者：
Cummings, Richard D.
中科院分区：
文献类型：
--
作者：
Stowell, Sean R.;Karmakar, Sougata;Cummings, Richard D.
细胞周转与凋亡细胞表面磷脂酰丝氨酸 (PS) 的暴露有关，导致其吞噬细胞识别和清除。但最近的研究表明，表面 PS 暴露并不总是与细胞凋亡相关。在这里，我们表明，人类半乳糖凝集素家族的聚糖结合蛋白（半乳糖凝集素-1、-2和-4）的几个成员以碳水化合物依赖性方式在激活的而非静息的人类中性粒细胞和几种白细胞细胞系中诱导PS暴露。 PS 暴露与活化中性粒细胞的凋亡无关。在用这些半乳糖凝集素处理的细胞系中，PS 的暴露是持续的，并且不会影响细胞活力。出乎意料的是，这些半乳糖凝集素与活化的T淋巴细胞良好结合，但不诱导PS暴露或细胞凋亡，这表明半乳糖凝集素的作用是细胞特异性的。这些结果表明半乳糖凝集素在独立于细胞凋亡调节白细胞周转方面具有新的免疫调节作用。 (c) 2007 年，美国血液学会
Cellular turnover is associated with exposure of surface phosphatildylserine (PS) in apoptotic cells, leading to their phagocytic recognition and removal. But recent studies indicate that surface PS exposure is not always associated with apoptosis. Here we show that several members of the human galectin family of glycan binding proteins (galectins-1, -2, and -4) induce PS exposure in a carbohydrate-dependent fashion in activated, but not resting, human neutrophils and in several leukocyte cell lines. PS exposure is not associated with apoptosis in activated neutrophils. The exposure of PS in cell lines treated with these galectins is sustained and does not affect cell viability. Unexpectedly, these galectins bind well to activated T lymphocytes, but do not induce either PS exposure or apoptosis, indicating that galectin's effects are cell specific. These results suggest novel immunoregulatory contribution of galectins in regulating leukocyte turnover independently of apoptosis. (c) 2007 by The American Society of Hematology