Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry
Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry
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DOI：
10.1016/j.neulet.2007.02.028
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发表时间：
2007-04-24
影响因子：
2.5
通讯作者：
Nagano, Akira
中科院分区：
文献类型：
--
作者：
Sawada, Tomokazu;Sano, Michio;Nagano, Akira
本研究的目的是利用免疫组织化学和酶联免疫吸附测定（ELISA）定量研究大鼠坐骨神经瓦勒变性和挤压损伤后再生过程中肿瘤坏死因子-α（TNF）和白介素-10（IL-10）免疫阳性细胞的纵向时间和空间变化。损伤后3天，挤压部位远端所有节段的TNF免疫阳性细胞数量达到峰值并显着增加。第 7 天，挤压部位远端所有节段的 TNF 免疫阳性细胞均减少，损伤后 14 天观察到显着减少。从第21天到第56天，TNF免疫阳性细胞的数量没有显着差异。 TNF 免疫阳性细胞的平均大小随着变性而显着变大。挤压伤后 1 天，IL-10 免疫阳性细胞的数量显着减少。 IL-10 免疫阳性细胞在第 3 天增加，恢复到对照水平。损伤后 7 天，观察到 IL-10 免疫阳性细胞数量显着增加。第 14 天之后，除了部分远端节段外，IL-10 免疫阳性细胞的数量也没有显着差异。第56天各节段IL-10免疫阳性细胞数无明显差异。ELISA检测IL-10蛋白水平与免疫组化结果相似。这些结果表明IL-10的显着变化发生在TNF的显着变化之前，并且IL-10可能是TNF变化的关键。 (c) 2007 Elsevier Ireland Ltd. 保留所有权利。
The purpose of this study was to investigate quantitatively the longitudinal temporal, spatial changes of the tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10) immunopositive cells during Wallerian degeneration and the following regeneration after crush injury in rat sciatic nerve using immunohistochemistry and enzyme linked immunosorbent assay (ELISA). The number of TNF-immunopositive cells reached its peak and increased significantly in all the segments distal to the crush site 3 days after injury. On Day 7, TNF-immunopositive cells decreased in all the segments distal to the crush site, and a significant decrease was observed 14 days after injury. From Day 21 to Day 56, there were no significant differences in the numbers of TNF-immunopositive cells. The average size of TNF immunopossitive cells became significantly larger with degeneration. The number of IL-10-immunopositive cells decreases significantly 1 day after crush injury. IL-10-immunopositive cells increased on Day 3, returning to control levels. Seven days after injury, a significant increase in the number of IL-10-immunopositive cells was observed. There was also no significant difference in the number of IL-10-immunopositive cells beyond Day 14 except for apart of distal segments. The number of IL-10-immunopositive cells showed no significant differences in all the segments on Day 56. The protein levels of IL-10 measured by ELISA were similar to the result of immunohistochemistry. These results suggest that the significant change in IL-10 occurred prior to the significant change in TNF and that IL-10 may be the key to the change in TNF. (c) 2007 Elsevier Ireland Ltd. All rights reserved.