MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A PUTATIVE MEMBER OF THE RAT OPIOID RECEPTOR GENE FAMILY THAT IS NOT A MU-OPIOID, DELTA-OPIOID OR KAPPA-OPIOID RECEPTOR-TYPE
MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A PUTATIVE MEMBER OF THE RAT OPIOID RECEPTOR GENE FAMILY THAT IS NOT A MU-OPIOID, DELTA-OPIOID OR KAPPA-OPIOID RECEPTOR-TYPE
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DOI：
10.1016/0014-5793(94)00561-3
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发表时间：
1994-06-27
期刊：
影响因子：
3.5
通讯作者：
GRANDY, DK
中科院分区：
文献类型：
--
作者：
BUNZOW, JR;SAEZ, C;GRANDY, DK
通过PCR和同源性筛选，克隆了一个新的G蛋白偶联受体。其推导的氨基酸序列与μ、δ和κ阿片受体总体上47%相同，并且在推定的跨膜结构域中64%相同。当在COS-7细胞中瞬时表达时，该受体不以高亲和力结合任何典型的μ、δ或κ阿片样物质受体配体。原位杂交分析表明，LC 132 mRNA在几个脑区，包括大脑皮质，丘脑，穹窿下器，缰核，下丘脑，中央灰质，中缝背核，蓝斑和脊髓背角的高表达。基于这种分布及其与μ、δ和κ阿片受体的高度同源性，提出LC 132是参与镇痛和疼痛感知的阿片受体家族的新成员。
A novel G protein-coupled receptor was cloned by PCR and homology screening. Its deduced amino acid sequence is 47% identical overall to the mu, delta and kappa opioid receptors and 64% identical in the putative transmembrane domains. When transiently expressed in COS-7 cells this receptor did not bind any of the typical mu, delta or kappa opioid receptor ligands with high affinity. In situ hybridization analysis revealed that LC132 mRNA is highly expressed in several rat brain areas, including the cerebral cortex, thalamus, subfornical organ, habenula, hypothalamus, central gray, dorsal raphe, locus coeruleus and the dorsal horn of the spinal cord. Based on this distribution and its high homology with the mu, delta and kappa opioid receptors, it is proposed that LC132 is a new member of the opioid receptor family that is involved in analgesia and the perception of pain.