Cytokeratin expression in epithelial cells isolated from the crypt and villus regions of the rodent small intestine.
Cytokeratin expression in epithelial cells isolated from the crypt and villus regions of the rodent small intestine.
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从啮齿动物小肠的隐窝和绒毛区域分离的上皮细胞中细胞角蛋白的表达。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
G. S. Evans
中科院分区：
文献类型：
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作者：
N. Flint;P. Pemberton;R. Lobley;G. S. Evans
小肠上皮的许多生理和结构特征在其从隐窝基部到绒毛尖端的运输过程中受到调节。这种隐窝-绒毛轴是研究细胞增殖和分化调控的重要模型。我们已经研究了细胞角蛋白的表达纯化上皮细胞从增殖（隐窝）和分化（绒毛）地区的这一组织。三个多肽被确定（细胞角蛋白8，18和19），以及第四，46 kDa的多肽与最近确定的细胞角蛋白20类似的电泳特性。这些分子的分布被发现变化沿着的隐窝绒毛轴，与细胞角蛋白18被限制到增生的隐窝和细胞角蛋白8和19表现出更均匀的分布。发现46 kDa组分主要在绒毛上皮内表达。虽然没有实质性的证据表明细胞角蛋白在上皮分化过程中的直接作用，这些数据表明，细胞角蛋白的差异表达与肠上皮分化的变化。
Many physiological and structural features of epithelium in the small intestine are regulated during their transit from the crypt base to the villus tip. This crypt-villus axis is an important model for the study of the regulation of cell proliferation and differentiation. We have investigated the expression of cytokeratins in purified epithelial cells from the proliferative (crypt) and differentiated (villus) regions of this tissue. Three polypeptides were identified (cytokeratins 8, 18 and 19) as well as a fourth, 46 kDa polypeptide with similar electrophoretic characteristics to the recently identified cytokeratin 20. The distribution of these molecules was found to vary along the crypt-villus axis, with cytokeratin 18 being restricted to the proliferative crypt and cytokeratins 8 and 19 demonstrating more uniform distributions. The 46 kDa component was found to be expressed predominantly within the villus epithelium. Although there is no substantial evidence of a direct role for cytokeratins in the process of epithelial differentiation, these data suggest that differential expression of cytokeratins is associated with changes in intestinal epithelial differentiation.