Localization-based super-resolution microscopy with an sCMOS camera Part II: Experimental methodology for comparing sCMOS with EMCCD cameras
Localization-based super-resolution microscopy with an sCMOS camera Part II: Experimental methodology for comparing sCMOS with EMCCD cameras
复制标题
使用 sCMOS 相机的基于定位的超分辨率显微镜第二部分：比较 sCMOS 与 EMCCD 相机的实验方法
DOI：
10.1364/oe.20.017741
复制
发表时间：
2012-07-30
期刊：
影响因子：
3.8
通讯作者：
Huang, Zhen-Li
中科院分区：
文献类型：
--
作者：
Long, Fan;Zeng, Shaoqun;Huang, Zhen-Li
目前，新开发的科学级互补金属氧化物半导体（Complementary Metal Oxide Semiconductor, sCMOS）相机能否成为定位超分辨率显微镜中首选的图像传感器，是业界和学术界争论的热点。为了帮助研究人员找到这个问题的答案，我们在这里报告了一种实验方法，用于定量比较弱光相机在单分子检测（通过图像信噪比表征）和定位（通过定位精度表征）方面的性能。我们发现，新推出的sCMOS相机在信号范围（15-12000光子/像素）上比流行的电子倍增电荷耦合器件（EMCCD）相机具有更好的成像性能，足以用于典型的基于定位的超分辨率显微镜。(C) 2012美国光学学会
Nowadays, there is a hot debate among industry and academic researchers that whether the newly developed scientific-grade Complementary Metal Oxide Semiconductor (sCMOS) cameras could become the image sensors of choice in localization-based super-resolution microscopy. To help researchers find answers to this question, here we reported an experimental methodology for quantitatively comparing the performance of low-light cameras in single molecule detection (characterized via image SNR) and localization (via localization accuracy). We found that a newly launched sCMOS camera can present superior imaging performance than a popular Electron Multiplying Charge Coupled Device (EMCCD) camera in a signal range (15-12000 photon/pixel) more than enough for typical localization-based super-resolution microscopy. (C) 2012 Optical Society of America