CpG island methylator phenotype-low (CIMP-low) colorectal cancer shows not only few methylated CIMP-high-specific CpG islands, but also low-level methylation at individual loci
CpG island methylator phenotype-low (CIMP-low) colorectal cancer shows not only few methylated CIMP-high-specific CpG islands, but also low-level methylation at individual loci
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DOI：
10.1038/modpathol.3800982
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发表时间：
2008-03-01
期刊：
影响因子：
7.5
通讯作者：
Ogino, Shuji
中科院分区：
文献类型：
--
作者：
Kawasaki, Takako;Ohnishi, Mutsuko;Ogino, Shuji
具有广泛启动子甲基化的CpG岛甲基甲基表型（CIMP或CIMP-HIGH）是大肠癌的独特表型。但是，CPG岛甲基化较少的CIMP-LOW的概念仍在发展。我们的目的是检查单个CPG岛中甲基化密度之间的甲基化密度是否在CIMP-LOW和CIMP-HIGH肿瘤之间有所不同。利用甲基景观技术和889种基于人群的大肠癌，我们在14个CpG岛上定量DNA甲基化（甲基化指数，甲基化参考百分比），包括8个CIMP-High特异性基因座（CACNA1G，CDKN2A，CDKN2A（P16），CRABP1，CRABP1，IGF2，IGF2，IGF2，IGF2，IGF2，IGF2，IGF2，IGF2 ，neurog1，runx3和socs1）。每个基因座的甲基化阳性被定义为甲基化指数>4。低级甲基化（甲基化指数> 0，= 6/8甲基化 - 阳性基因座）和416 CIMP-0肿瘤（0/8甲基化阳性基因座）（P
The CpG island methylator phenotype (CIMP or CIMP-high) with widespread promoter methylation is a distinct phenotype in colorectal cancer. However, the concept of CIMP-low with less extensive CpG island methylation is still evolving. Our aim is to examine whether density of methylation in individual CpG islands was different between CIMP-low and CIMP-high tumors. Utilizing MethyLight technology and 889 population-based colorectal cancers, we quantified DNA methylation (methylation index, percentage of methylated reference) at 14 CpG islands, including 8 CIMP-high-specific loci (CACNA1G, CDKN2A (p16), CRABP1, IGF2, MLH1, NEUROG1, RUNX3 and SOCS1). Methylation positivity in each locus was defined as methylation index > 4. Low-level methylation (methylation index > 0, = 6/8 methylation-positive loci) and 416 CIMP-0 tumors (0/8 methylation-positive loci) (P