Serological and molecular studies on subclinical hepatitis E virus infection using periodic serum samples obtained from healthy individuals
Serological and molecular studies on subclinical hepatitis E virus infection using periodic serum samples obtained from healthy individuals
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DOI：
10.1002/jmv.20393
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发表时间：
2005-08-01
影响因子：
12.7
通讯作者：
Okamoto, H
中科院分区：
文献类型：
--
作者：
Mitsui, T;Tsukamoto, Y;Okamoto, H
本文对健康人戊型肝炎病毒（HEV）亚临床感染进行了血清学和分子生物学研究。2004年3月至4月筛选时采集的血清样本（或退休前）从266名医务人员（35名男性，231名女性），（平均标准差，范围，0.3-35.1）在日本的一家城市医院工作了10年，并对在开始工作时从这些工作人员收集的血清样品进行伊加，IgM，和抗HEV的IgG抗体（抗HEV）。总体而言，6例受试者（2.3%）在筛选时抗-HEV IgG检测结果呈阳性;其中，4例受试者（1.5%）在入职时抗-HEV IgG已呈阳性，2例受试者（0.8%）在入职后发生血清转换。从两名血清转化受试者中定期采集血清样本，检测HEV抗体和HEV RNA。这两名受试者分别于1978年或2003年抗HEV IgG阳性，丙氨酸氨基转移酶（ALT）水平无明显升高，并持续血清阳性直至筛选日期。虽然抗-HEVIgM是不可检测的两个主题，一个是日本本土的基因型3的HEV株瞬时感染，另一个是阳性的抗-伊加。目前的研究表明，即使是一个人与亚临床HEV感染的ALT升高的情况下，短暂的病毒血症的证据和抗-伊加检测可能是有用的血清学诊断最近亚临床HEV感染。(c)2005 Wiley-Liss，Inc.
Subdinical hepatitis E virus (HEV) infection among healthy individuals was studied serologically and molecularly. Serum samples collected at screening between March and April 2004 (or just before retirement) from 266 medical staff members (35 males, 231 females) who had been working for 8.8 +/- 8.5 (mean standard deviation, range, 0.3-35.1) years in a city hospital in Japan and serum samples that had been collected from these staff members at the start of employment were tested for IgA, IgM, and IgG antibodies to HEV (anti-HEV) by in-house enzyme-linked immunosorbent assays. Overall, six subjects (2.3%) tested positive for anti-HEV IgG at the screening; among them, four subjects (1.5%) had already been positive for anti-HEV IgG at the start of employment and two subjects (0.8%) seroconverted after initiation of employment. Periodic serum samples that had been collected from the two seroconverted subjects were tested for HEV antibodies and HEV RNA. The two subjects became positive for anti-HEV IgG in 1978 or 2003, respectively, with no discernible elevation in alanine aminotransferase (ALT) level, and continued to be seropositive up through the screening date. Although anti-HEV IgM was not detectable in the two subjects, one was infected transiently with Japan-indigenous HEV strain of genotype 3 and the other was positive transiently for anti-HEV IgA. The present study indicates that even an individual with subdinical HEV infection had evidence of transient viremia in the absence of ALT elevation and that anti-HEV IgA detection may be useful for serological diagnosis of recent subdinical HEV infection. (c) 2005 Wiley-Liss, Inc.