Immunomagnetic separation of tumor initiating cells by screening two surface markers.
Immunomagnetic separation of tumor initiating cells by screening two surface markers.
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DOI：
10.1038/srep40632
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发表时间：
2017-01-11
影响因子：
4.6
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Sun C;Hsieh YP;Ma S;Geng S;Cao Z;Li L;Lu C
分离肿瘤起始细胞（TIC）通常需要筛选多种表面标志物，有时具有相反的偏好。这对使用基于珠粒的免疫磁性分离（IMS）产生了挑战，所述基于珠粒的免疫磁性分离通常基于一种丰富的标记物富集细胞。在这里，我们提出了一种新的策略，允许通过IMS分离CD 44 +/CD 24 − TIC，包括抗CD 44抗体包被的磁珠和抗CD 24抗体包被的磁珠（称为双珠IMS）。用我们的方法富集的细胞显示出TIC标记物表达的显著增强（通过流式细胞术检查）和提高的肿瘤球形成效率。我们的方法将IMS的应用扩展到以多种标记物为特征的细胞亚群。
Isolating tumor initiating cells (TICs) often requires screening of multiple surface markers, sometimes with opposite preferences. This creates a challenge for using bead-based immunomagnetic separation (IMS) that typically enriches cells based on one abundant marker. Here, we propose a new strategy that allows isolation of CD44+/CD24− TICs by IMS involving both magnetic beads coated by anti-CD44 antibody and nonmagnetic beads coated by anti-CD24 antibody (referred to as two-bead IMS). Cells enriched with our approach showed significant enhancement in TIC marker expression (examined by flow cytometry) and improved tumorsphere formation efficiency. Our method will extend the application of IMS to cell subsets characterized by multiple markers.