Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division.
Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division.
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DOI：
10.7554/elife.20977
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发表时间：
2016-11-25
期刊：
影响因子：
7.7
通讯作者：
Yamashita YM
中科院分区：
文献类型：
--
作者：
Chen C;Inaba M;Venkei ZG;Yamashita YM
不对称干细胞分裂通常伴随着母体和女儿中心体的刻板遗传。然而，干细胞中心体是否以及如何受到独特的调控，以及这种调控如何影响干细胞的命运，目前尚不清楚。本研究发现，在果蝇雄性生殖系干细胞（GSCs）中，Klp10A是第一个富集在干细胞中心体中的蛋白，它是一种微管解聚驱动蛋白13家族的驱动蛋白。klp10A的缺失导致GSCs中母中心体的异常伸长，表明存在干细胞特异性中心体调控程序。随着母中心体的伸长，GSCs形成不对称纺锤体，其中伸长的母中心体组织的半纺锤体比另一个大得多。这导致细胞大小不对称，产生较小的分化子细胞。我们提出klp10A的功能是抵消不希望的不对称，这种不对称可能是干细胞成功分裂所必需的不对称的副产品。DOI: http://dx.doi.org/10.7554/eLife.20977.001
Asymmetric stem cell division is often accompanied by stereotypical inheritance of the mother and daughter centrosomes. However, it remains unknown whether and how stem cell centrosomes are uniquely regulated and how this regulation may contribute to stem cell fate. Here we identify Klp10A, a microtubule-depolymerizing kinesin of the kinesin-13 family, as the first protein enriched in the stem cell centrosome in Drosophila male germline stem cells (GSCs). Depletion of klp10A results in abnormal elongation of the mother centrosomes in GSCs, suggesting the existence of a stem cell-specific centrosome regulation program. Concomitant with mother centrosome elongation, GSCs form asymmetric spindle, wherein the elongated mother centrosome organizes considerably larger half spindle than the other. This leads to asymmetric cell size, yielding a smaller differentiating daughter cell. We propose that klp10A functions to counteract undesirable asymmetries that may result as a by-product of achieving asymmetries essential for successful stem cell divisions. DOI: http://dx.doi.org/10.7554/eLife.20977.001