Higher ROS scavenging ability and plasma membrane H+-ATPase activity are associated with potassium retention in drought tolerant tea plants
Higher ROS scavenging ability and plasma membrane H+-ATPase activity are associated with potassium retention in drought tolerant tea plants
复制标题
较高的 ROS 清除能力和质膜 H-ATP 酶活性与耐旱茶树的钾保留有关
DOI：
10.1002/jpln.202000007
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发表时间：
2020
影响因子：
2.5
通讯作者：
Wang Xiaochun
中科院分区：
文献类型：
--
作者：
Zhang Xianchen;Wu Honghong;Chen Jingguang;Chen Linmu;Chang Na;Ge Gaofei;Wang Xiaochun
干旱是导致茶叶产量和品质下降的主要限制因子之一。我们以前的研究表明，K+潴留是缓解茶树干旱损伤的关键因素。然而，其潜在机制仍不清楚。结果表明，耐旱茶树品种中茶108的质膜H+-ATPase活性高于耐旱茶树品种软枝乌龙，而质膜负电位低于耐旱茶树品种软枝乌龙。耐旱茶树不仅具有上级的保钾能力和较高的质膜H+-ATPase活性，而且活性氧（ROS）的积累也低于敏感茶树。此外，外源供应Na 3VO 4（质膜H+-ATP酶抑制剂）和H2 O2（模拟质外体ROS）导致干旱敏感茶树叶肉中的K+流出量显著高于未处理的茶树。因此，较高的ROS清除能力和质膜H+-ATPase活性是耐旱茶树较高K+存留率的主要原因。
Drought is one of the main limiting factors resulting in the reduction of yield and quality of tea production. Our previous studies indicated that K+retention was a key factor associated with alleviating drought‐induced damage in tea plants. However, the underlying mechanisms are still unclear. In the present study, drought tolerant tea plant (variety Zhongcha108) exhibited higher plasma membrane (PM) H+‐ATPase activity and lower negative membrane potential compared with the drought sensitive tea plant (variety Ruanzhiwulong). In addition to superior mesophyll K+retention ability and higher PM H+‐ATPase activity, the drought tolerant tea plant exhibited lower accumulation of reactive oxygen species (ROS) than the sensitive one. Moreover, exogenous supply of Na3VO4(plasma membrane H+‐ATPase inhibitor) and H2O2(mimicking apoplastic ROS) resulted in a significantly higher K+efflux in the mesophyll of the drought sensitive tea plant than the non‐treated one. Taken together, the higher ROS scavenging ability and plasma membrane H+‐ATPase activity were major factors contributing to the higher K+retention in drought tolerant tea plants.