Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins
Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins
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DOI：
10.1104/pp.122.3.803
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发表时间：
2000-03-01
期刊：
影响因子：
7.4
通讯作者：
Robinson, SP
中科院分区：
文献类型：
--
作者：
Davies, C;Robinson, SP
我们使用差分筛选将与成熟的成熟相关的cDNA分离出与设拉子葡萄（Vitis Vinifera L.）浆果cDNA库。在成熟发作时，未成熟果实中不存在的许多cDNA的mRNA水平迅速增加。其中一些克隆的推定翻译产物在其他物种中具有与细胞壁结构有关的同源物。其中包括四种富含脯氨酸的蛋白质，这是一种类似于某些果胶甲基酯酶的非催化，N末端结构域的小蛋白质，以及另外两种富含谷氨酸的蛋白。其余的克隆编码了推定的应激反应蛋白。其中包括两种像蛋白质，一种金属硫蛋白，转录因子，一种细胞色素P450酶以及其他物种中水，糖和/或冷应激诱导的蛋白质。被认为与细胞壁结构有关的葡萄cDNA的许多同源物或与压力相关的反应也在其他植物中以发育方式积累。这可能表明，葡萄mRNA会响应于诸如储存高浓度糖和快速细胞膨胀等应力的响应，或者它们可能作为成熟发展计划的一部分积累。
We used differential screening to isolate ripening-associated cDNAs from a Shiraz grape (Vitis vinifera L.) berry cDNA library. A rapid increase in the mRNA levels of a number of cDNAs not present in unripe fruit occurred in grape berries at the onset of ripening. The putative translation products of some of these clones had homologs in other species that are involved in cell wall structure. These included four proline-rich proteins, a small protein that is similar to the non-catalytic, N-terminal domain of some pectin methylesterases, and two other glutamate-rich proteins. The remainder of the clones encoded putative stress response proteins. These included two thaumatin like proteins, a metallothionein, a transcription factor, a cytochrome P450 enzyme, and proteins induced by water, sugar, and/or cold stress in other species. Many of the homologs of the grape cDNAs thought to be involved in cell wall structure or stress-related responses also accumulate in a developmental manner in other plants. This may indicate that the grape mRNAs accumulate in response to stresses such as the storage of high concentrations of sugars and rapid cell expansion, or they may accumulate as part of the ripening developmental program.