Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer
Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer
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miR-193a-5p 的下调与结直肠癌淋巴结转移和不良预后相关
DOI：
10.3748/wjg.v20.i34.12241
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发表时间：
2014-09-14
影响因子：
4.3
通讯作者：
Gu, Jin
中科院分区：
文献类型：
--
作者：
Zhang, Peng;Ji, Deng-Bo;Gu, Jin
目的：方法：收集经福尔马林固定的结直肠癌组织标本304例（69例结直肠癌与正常组织配对，55例III期结直肠癌原发灶与配对淋巴结，56例II期结直肠癌原发灶），分析miR-193 a-5 p与结直肠癌淋巴结转移及术后生存的关系。通过实时定量逆转录聚合酶链反应测定miR-193 a-5 p在正常粘膜、原发癌和转移性淋巴结病变中的相对表达水平。结果：miR-193 a-5 p在结直肠癌组织中的相对表达水平显著低于正常黏膜组织（P = 0.0060），其表达水平与结直肠癌淋巴结转移、临床病理因素和患者生存率之间存在相关性。有淋巴结转移的原发性CRC组织中miR-193 a-5 p的表达水平低于无淋巴结转移的原发性CRC组织（P = 0.0006），并且miR-193 a-5 p的表达降低与淋巴结转移分期晚期相关（P = 0.0007）。Kaplan-Meier分析显示，miR-193 a-5 p低表达患者的无病生存期（DFS）降低（P = 0.0026），总生存期（OS）较差（P = 0.0003）。有趣的是，对于淋巴结转移患者组，miR-193 a-5 p表达也与生存相关。miR-193 a-5 p低表达的患者DFS降低（P = 0.0262），OS较差（P = 0.0230）。结论：miR-193 a-5 p表达下调与结直肠癌淋巴结转移和生存率相关。miR-193 a-5 p可能是CRC诊断、转移和预后预测的一个有用的生物标志物。(C)2014百世登出版集团股份有限公司All rights reserved.
AIM: To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer (CRC) patients.METHODS: A total of 304 formalin-fixed, paraffin-embedded specimens (69 paired cancer and normal tissues, 55 primary tumors of stage III CRC and matched lymph nodes, and 56 primary tumors of stage II CRC) were included in this study. The relative expression levels of miR-193a-5p in the normal mucosa, primary cancer, and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction. We evaluated the association of its expression with colorectal cancer lymph node metastasis, clinicopathological factors, and patient survival.RESULTS: The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa (P = 0.0060). The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases (P = 0.0006), and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage (P = 0.0007). Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival (DFS) (P = 0.0026) and poor overall survival (OS) (P = 0.0003). Interestingly, for the group of patients with lymph node metastases, miR-193a-5p expression was also related to survival. Patients with low miR-193a-5p expression had decreased DFS (P = 0.0262) and poor OS (P = 0.0230). Moreover, multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS.CONCLUSION: Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC. miR-193a-5p may be a useful biomarker for CRC diagnosis, metastasis and prognosis prediction. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.