Automated image analysis of bacterial colony growth as a tool to study individual lag time distributions of immobilized cells
Automated image analysis of bacterial colony growth as a tool to study individual lag time distributions of immobilized cells
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DOI：
10.1016/j.mimet.2005.08.007
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发表时间：
2006-05-01
影响因子：
2.2
通讯作者：
Augustin, J. -C.
中科院分区：
文献类型：
--
作者：
Guillier, L.;Pardon, P.;Augustin, J. -C.
提出了一种测定固定化细菌个体滞后时间分布的方法。该方法是基于细菌菌落生长的图像分析。从形成宏观可见菌落所需的检测时间（T-d）的分布中检索滞后分布。利用这种方法，测定了奶酪污染过程中遇到的两种繁殖条件下单核增生李斯特菌细胞在琼脂上的滞后分布。给出了结果，并与基于检测肉汤浊度时间的既定方法得到的滞后分布进行了比较。提出了一种在不知道生长速率的情况下，从肉汤和琼脂中提取滞后菌的方法。为了不使滞后菌在琼脂上的分布产生偏倚，对菌落空间间隔对菌落生长速率的影响进行了量化。两种不同胁迫下的滞后分布的均值和标准差在肉汤和琼脂上相似。极值II型分布最适合滞后分布的不同数据集。(c) 2005 Elsevier B.V.版权所有
A method to determine the individual lag time (lag) distributions of immobilized bacteria was presented. The method was based on the image analysis of the bacterial colony growth. The lag distributions were retrieved from the distributions of the detection times (T-d) required to form macroscopically visible colonies. Using this method, the lag distributions on agar for Listeria monocytogenes cells previously subjected to two situations reproducing conditions encountered during the contamination of cheese, were determined. The results were presented and compared with lag distributions obtained with an established method based on the time to detection of turbidity in broth. An original method to retrieve lag in broth and agar without any knowledge of the growth rate was also proposed. In order not to bias the distributions of lag on agar the impact of spatial separation between colonies on colony growth rates was quantified. Means and standard deviations of lag distributions for the two different stresses were found to be similar in broth and on agar. Extreme Value type II distributions fitted the best the different datasets of lag distributions. (c) 2005 Elsevier B.V. All rights reserved.