Immunoglobulin VH gene expression in human B cell lines and tumors: biased VH gene expression in chronic lymphocytic leukemia.
Immunoglobulin VH gene expression in human B cell lines and tumors: biased VH gene expression in chronic lymphocytic leukemia.
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人类 B 细胞系和肿瘤中的免疫球蛋白 VH 基因表达：慢性淋巴细胞白血病中的偏向 VH 基因表达。
DOI：
10.1093/intimm/1.4.362
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发表时间：
1989
影响因子：
4.4
通讯作者：
Alt,FW
中科院分区：
文献类型：
--
作者：
Logtenberg,T;Schutte,ME;Inghirami,G;Berman,JE;Gmelig-Meyling,FH;Insel,RA;Knowles,DM;Alt,FW
我们研究了VH基因利用率在一组单克隆EB病毒（EBV）转化的B细胞系来自成人和胎儿组织，以及在单克隆B细胞从新鲜的慢性淋巴细胞白血病（CLL）样本。结果显示，来自胎儿和成人组织的分泌IgM的EBV细胞系利用来自特定家族的VH基因，大致与估计的家族大小成比例，表明胎儿和成人器官中的IgM阳性B细胞库相对于VH基因家族是“标准化的”。相反，我们在CLLs中发现了VH基因表达的高度blased模式。这些研究结果的意义进行了讨论的背景下，可能参与正常的B细胞库的发展和CLL的前体的恶性转化的过程中的机制。
We have studied frequencies of VHgene utilization in a panel of monoclonal Epstein–Barr virus (EBV)-transformed B cell lines derived from human adult and fetal tissues as well as in monoclonal B cells obtained from fresh chronic lymphocytic leukemia (CLL) samples. The results show that IgM-secreting EBV cell lines from both fetal and adult tissues utilize VHgenes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of slgM-positlve B cells in both fetal and adult organs is ‘normalized’ with respect to the VHgene family. In contrast, we find a highly blased pattern of VHgene expression in CLLs. The significance of these findings is discussed in the context of mechanisms that could be involved in normal B cell repertoire development and in the process of malignant transformation of precursors of CLL.