Mullerian inhibiting substance in the adult rat ovary during various stages of the estrous cycle.
Mullerian inhibiting substance in the adult rat ovary during various stages of the estrous cycle.
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DOI：
10.1210/endo-125-2-1060
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发表时间：
1989-08
期刊：
影响因子：
4.8
通讯作者：
Shigeru Ueno;Tatsuo Kuroda;D. Maclaughlin;R. Ragin;T. Manganaro;P. K. Donahoe
中科院分区：
文献类型：
--
作者：
Shigeru Ueno;Tatsuo Kuroda;D. Maclaughlin;R. Ragin;T. Manganaro;P. K. Donahoe
用免疫组织化学方法，在动情期、动情后期、动情间期和动情前期大鼠生长中的腔前卵泡颗粒细胞、有腔卵泡的卵丘和腔周颗粒细胞中检测到苗勒管抑制物质（MIS）。黄体和闭锁卵泡均未被MIS染色。在发情前期，发生了显着的变化，在大的排卵前窦状卵泡，在一天的早期表现出强烈的MIS特异性染色的颗粒细胞位于靠近卵母细胞，然而，在2300小时，排卵前，当生殖囊泡被挤出，表明恢复减数分裂，MIS染色减弱。当晚排卵前阶段的形态学实验中创建的26日龄的未成熟卵巢刺激48小时前与hCG，强烈染色的颗粒细胞周围的卵母细胞从未经处理的卵巢中丢失在卵丘细胞的hCG治疗的动物。MIS染色的这种时间模式和先前的证明，MIS可以抑制未处理的未成熟大鼠卵母细胞的体外减数分裂表明，这种回归，以及在胎儿睾丸中的特点，可能在卵巢中作为一个调节器的卵母细胞成熟和卵泡发育在成年生殖周期。
Mullerian inhibiting substance (MIS) in the adult rat ovary can be detected by immunohistochemistry in the granulosa cells of growing preantral follicles and in the cumulus oophorus and periluminal granulosa cells of antral follicles in estrus, metestrus, diestrus, and proestrus. Neither the corpus luteum nor atreitic follicles stained for MIS. During proestrus, dramatic changes occurred in the large preovulatory antral follicles, which early in the day manifest intense MIS-specific staining in the granulosa cells located close to the oocyte, however, at 2300 h, just before ovulation when the germinal vesicle was extruded to indicate resumed meiotic division, MIS staining waned. When the morphology of the late preovulatory stage was created experimentally in 26-day-old immature ovaries by stimulating 48 h earlier with hCG, the intense staining of the granulosa cells surrounding the oocytes from untreated ovaries was lost in the cumulus cells of such hCG-treated animals. This temporal pattern of MIS staining and the prior demonstration that MIS could inhibit in vitro meiosis of oocytes from untreated immature rats suggest that this regressor, well characterized in the fetal testis, might function in the ovary as a regulator of oocyte maturation and follicular development during the adult reproductive cycle.