Placental 11B-Hydroxysteroid Dehydrogenase Type 2 mRNA Levels in Intrauterine Growth Restriction versus Small-for-Gestational-Age Fetuses
Placental 11B-Hydroxysteroid Dehydrogenase Type 2 mRNA Levels in Intrauterine Growth Restriction versus Small-for-Gestational-Age Fetuses
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DOI：
10.1159/000437139
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发表时间：
2015-08
影响因子：
2.2
通讯作者：
M. Dolores G�mez-Roig;E. Mazarico;Daniela C�rdenas;M. Teresa Fernandez;Marta D�az;B. Ruiz de Gauna;A. Vela;Eduard Gratac�s;F. Figueras
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文献类型：
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作者：
M. Dolores G�mez-Roig;E. Mazarico;Daniela C�rdenas;M. Teresa Fernandez;Marta D�az;B. Ruiz de Gauna;A. Vela;Eduard Gratac�s;F. Figueras
目的：探讨胎盘11B-羟基类固醇脱氢酶2(11B-HSD-2)在胎儿宫内发育受限(IUGR)和小于胎龄儿(SGA)胎盘组织中的表达。材料和方法：测定胎盘组织中11B-HSD-2mRNA的表达水平，包括出生体重(SGA)和胎龄匹配的适胎儿(AGA)。SGA分为IUGR(出生体重第3百分位数或第10百分位数子宫动脉多普勒或脑胎盘比值异常)或非IUGR SGA。提取RNA后，用逆转录和定量聚合酶链式反应检测mRNA的表达水平。结果：共分析38例胎盘，其中AGA 20例，SGA 18例。在SGA妊娠中，有13例符合IUGR标准。IUGR组11B-HSD-2活性[0.105(SD 0.328)]显著低于非IUGR SGA[0.304(SD 0.261)；p=0.018]和AGA[0.294(SD 0.328)；P=0.001]。在调整了潜在的混杂因素(如吸烟或母亲皮质醇水平)后，这些差异仍然显著。非IUGR SGA和AGA之间的活性水平没有显著差异。讨论：与SGA和正常生长的胎儿相比，IUGR胎儿的11B-HSD-2活性降低。这一发现为开发新的胎盘生物标志物用于胎儿矮小的表型特征提供了机会。
Introduction: The objective of this study was to evaluate placental 11B-hydroxysteroid dehydrogenase type 2 (11B-HSD-2) mRNA levels in intrauterine growth-restricted fetuses (IUGR) as compared with small-for-gestational-age (SGA) fetuses according to clinical criteria. Material and Methods: Placental levels of 11B-HSD-2 mRNA levels were measured in SGA (birth weight <10th centile) and gestational-age-matched, appropriate-for-gestational-age (AGA) births. SGA was classified as IUGR (birth weight <3rd centile or <10th percentile with abnormal uterine artery Doppler or cerebroplacental ratio) or non-IUGR SGA. After RNA extraction, mRNA levels were determined by reverse transcription and quantitative PCR. Results: A total of 38 placentas were analyzed (20 AGA and 18 SGA). Among the SGA pregnancies, 13 qualified as IUGR. The activity of 11B-HSD-2 in IUGR pregnancies [0.105 (SD 0.328)] was significantly reduced compared to non-IUGR SGA [0.304 (SD 0.261); p = 0.018] and AGA [0.294 (SD 0.328); p = 0.001]. These differences remained significant after adjusting for potential confounders (such as smoking or maternal cortisol levels). Activity levels did not significantly differ between non-IUGR SGA and AGA. Discussion: IUGR fetuses had reduced 11B-HSD-2 activity in comparison with SGA and normally grown fetuses. This finding provides opportunities to develop new placental biomarkers for the phenotypic characterization of fetal smallness.