High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
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DOI：
10.1038/nchembio732
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发表时间：
2005-10-01
影响因子：
14.8
通讯作者：
Fishman, MC
中科院分区：
文献类型：
--
作者：
Burns, CG;Milan, DJ;Fishman, MC
为了提高斑马鱼胚胎表型性状评分的便利性和吞吐量，我们开发了一种利用表达绿色荧光蛋白的转基因胚胎在心肌中的自动荧光显微镜进行心率自动微孔分析的方法。该方法在很大的线性动态范围内高效、准确地测量心率，并快速表征小分子引起的心率变化的剂量依赖性和动力学特征。这是第一次对完整脊椎动物的器官功能进行高通量微孔分析。
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.