Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis
Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis
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DOI：
10.1242/dev.01555
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发表时间：
2005-01-01
期刊：
影响因子：
4.6
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Chuma, S;Kanatsu-Shinohara, M;Shinohara, T
原始生殖细胞(PGC)来源于小鼠体内的一组多能上皮细胞。然而，对于PGCs何时以及如何获得分化为功能性生殖细胞的能力，同时保持分化为多潜能胚胎生殖细胞和畸胎癌的潜力，人们知之甚少。在这项研究中，我们证明了上皮母细胞和生殖细胞在移植到代孕不育小鼠的生精小管后，可以建立精子发生的集落。此外，我们通过显微受精获得了正常生育的后代，使用的精子或精子细胞来源于早在阴蒂后8.5天的胎儿的生殖细胞。因此，胎儿男性生殖细胞的发育是非常灵活的，并且成熟的过程。从外胚层细胞通过生殖细胞到生后精原细胞，可以发生在生后睾丸环境中。原始生殖细胞移植技术也为评估生殖细胞的发育潜力提供了一种新的工具。比如那些在体外进行操作或从含有致命突变的胚胎中恢复的基因。
Primordial germ cells (PGCs) are derived from a population of pluripotent epiblast cells in mice. However, little is known about when and how PGCs acquire the capacity to differentiate into functional germ cells, while keeping the potential to derive pluripotent embryonic germ cells and teratocarcinomas. In this investigation, we show that epiblast cells and PGCs can establish colonies of spermatogenesis after transfer into postnatal seminiferous tubules of surrogate infertile mice. Furthermore, we obtained normal fertile offspring by microinsemination using spermatozoa or spermatids derived from PGCs harvested from fetuses as early as 8.5 days post coitum. Thus, fetal male germ cell development is remarkably flexible, and the maturation process. from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques mill also provide a novel tool to assess the developmental potential of PGCs. such as those manipulated in vitro or recovered from embryos harboring lethal mutations.