Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses
Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses
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DOI：
10.1016/s0304-4017(02)00091-2
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发表时间：
2002-07-29
影响因子：
2.6
通讯作者：
Ortega-Mora, LM
中科院分区：
文献类型：
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作者：
Alvarez-García, G;Pereira-Bueno, J;Ortega-Mora, LM
研究了感染新孢子虫的小母牛和奶牛以及流产胎儿对新孢子虫速殖子抗原识别的不同方面。评估了抗原识别模式以及 IFAT 滴度与检测到的新孢子虫抗原数量之间的关系。此外，在正常奶牛和流产奶牛中，参与针对感染的体液免疫反应的速殖子抗原也在整个怀孕期间进行了表征。对来自新孢子虫流产胎儿的 13 份胸腔和/或腹腔液体和来自新孢子虫感染流产奶牛的 33 份血清进行了速殖子抗原识别的比较。在怀孕期间流产或未流产胎儿 (7) 或未流产 (14) 的感染新孢子虫的小母牛和奶牛中研究了新孢子虫抗原识别的动力学。根据识别的频率和强度，已经描述了四种 IDAs-17-18、34-35、37 和 60-62 kDa 抗原。此外，两个年龄组的蛋白质印迹结果和 IFAT 滴度之间也存在相关性。关于流产或未流产的血清阳性奶牛在整个怀孕期间的抗原识别，文献中描述的整个怀孕期间的抗体波动可能是由于特定抗原（尤其是 17-18 kDa 抗原）的识别强度和频率的差异所致。我们强调 17-18 kDa 抗原在牛新孢子虫感染的血清学诊断中发挥的重要作用，因为它在 100% 的动物中被强烈检测到。
Different aspects of Neospora tachyzoite antigen recognition by Neospora-infected heifers and cows and aborted foetuses were studied. The pattern of antigen recognition and the relationship between IFAT titres and number of Neospora antigens detected, were evaluated. In addition, the tachyzoite antigens involved in the humoral immune response developed against infection in normal cows and cows that aborted were also characterised throughout pregnancy. Comparison of tachyzoite antigen recognition was carried out in 13 thoracic and/or abdominal fluids from Neospora aborted foetuses and 33 sera from Neospora infected cows that had aborted. The kinetics of Neospora-antigen recognition was studied in Neospora-infected heifers and cows that had aborted foetuses (7) or not (14) during pregnancy.Based on the frequency and intensity of recognition, four IDAs-17-18, 34-35, 37 and 60-62 kDa antigens-have been described. Moreover, a correlation was found between Western blot results and IFAT titres in both age groups. In relation to antigen recognition throughout pregnancy by seropositive cows that had aborted or not, the antibody fluctuations throughout pregnancy described in the literature could be due to differences in the intensity and frequency of recognition of particular antigens, especially the 17-18 kDa antigen. We emphasize the important role that the 17-18 kDa antigen could play in the serological diagnosis of Neospora infection in cattle as this was intensely detected in 100% of the animals.