Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma
Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma
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DOI：
10.1016/j.rmed.2007.04.010
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发表时间：
2007-09-01
影响因子：
4.3
通讯作者：
Janciauskiene, Sabina M.
中科院分区：
文献类型：
--
作者：
Hollander, Camilla;Sitkauskiene, Brigita;Janciauskiene, Sabina M.
背景：呼吸道炎症与炎症反应物的表达和释放增加有关，炎症反应物调节细胞迁移、激活和脱颗粒的过程。本研究的目的是定量检测哮喘和慢性阻塞性肺疾病(COPD)患者BAL液和血清中趋化因子(IL-8)、分泌性白细胞蛋白酶抑制物(SLPI)、可溶性细胞间黏附分子-1(sICAM-1)和sCD14水平，以此作为哮喘和慢性阻塞性肺疾病(COPD)患者炎症和免疫反应的替代标志物。方法：用市售的酶联免疫试剂盒检测13例哮喘和25例COPD患者BAL液和BAL液中的生物标志物。结果：哮喘和COPD组BAL液中IL-8和SLPI浓度显著高于血清BAL液中sICAM-1和sCD14水平明显低于血清。在这4个生物标志物中，只有COPD患者的BAL IL-8高于哮喘(P<；0.05)。两组BALIL-8与SLPI呈正相关(r=0.577，P<0.01；r=0.589，P<0.05)。COPD患者BALsICAM-1与sCD14呈正相关(r=0.576，P<0.01)，哮喘患者BALsICAM-1与FEV、FVC呈正相关(r=0.418，P<0.01)。此外，哮喘患者血清SLPI与sCD14呈正相关(r=0.688，P<0.01)，sICAM-1与FEV1/FVC呈负相关(r=-0.582，P<0.05)。结论：在分析特定生物标志物时，选择正确的生物体液是非常重要的，而且在4项检测的生物标志物中，只有慢性阻塞性肺疾病患者的BALIL-8高于哮喘患者。2007年由爱思唯尔有限公司出版。
Background: Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time.Methods: Biomarkers in serum and BAL fluid from asthma (n = 13) and COPD (n = 25) patients were measured using commercially available ELISA kits.Results: We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P < 0.05). In both groups, BAL IL-8 correlated with SLPI (r = 0.577, P < 0.01 and r = 0.589, P < 0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r = 0.576, P < 0.01), while in asthma patients BAL sICAM-1 correlated with FEV,/FVC (r= 0.418, P < 0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P < 0.01) and serum sICAM-1 negatively correlated with FEV1/FVC (r= -0.582, P < 0.05).Conclusion: Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma. 2007 Published by Elsevier Ltd.