Impedimetric biosensor of Norovirus with low variance using simple bioconjugation on conductive polymer-Au nanocomposite
Impedimetric biosensor of Norovirus with low variance using simple bioconjugation on conductive polymer-Au nanocomposite
复制标题
使用导电聚合物-Au纳米复合材料上的简单生物共轭构建低方差的诺如病毒阻抗生物传感器
DOI：
10.1016/j.snb.2022.132390
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Park Enoch Y.
中科院分区：
文献类型：
--
作者：
Nasrin Fahmida;Khoris Indra Memdi;Chowdhury Ankan Dutta;Boonyakida Jirayu;Park Enoch Y.
电化学生物传感器在健康监测领域的指数增长表明在不久的将来应用的巨大可能性。然而，所制造的电极是不稳定的，具有高变异系数，即使实现了目标病毒的高灵敏度检测。在这项研究中，我们已经开发出一种简单的方法，传感器电极的制造已被用于电化学阻抗检测。已通过链霉亲和素生物素化制备了修饰的传感器，以制备高度稳定的基质，其产生用于诺如病毒检测的灵敏和可靠的响应，具有60 Ag/mL的低检测限。传感器电极的稳定基质及其优化的生物偶联条件使检测结果一致，方差值小于10%，证实了传感器在应用方面的优越性。此外，传感器的特异性已与其他病毒和传感介质进行了比较，由于传感器表面上的抗体的特异性结合，这是非常令人满意的。该传感器的灵敏度在临床分离的诺如病毒进行了比较，实时定量逆转录-聚合酶链反应（RT-qPCR），发现是令人满意的，其实际应用。传感器的制造和检测机制是直接和快速的，使其具有显着的优势，比其他电化学方法，其可能升级的试剂盒开发。
The exponential growth of electrochemical biosensors in the health monitoring sector indicates the enormous possibility of application in the near future. However, the fabricated electrodes are unstable with a high coefficient of variation, even though achieving highly sensitive detection of the target virus. In this study, we have developed a simple method of sensor electrode fabrication that has been used for electrochemical impedimetric detection. The modified sensor has been prepared by streptavidin biotinylation to make a highly stable matrix that generates a sensitive and reliable response for Norovirus detection with a low detection limit of 60 ag/mL. The stable matrix of the sensor electrode and its optimized condition for bioconjugation make a consistent detection result with a low variance value of less than 10 %, confirming the superiority of the sensor in terms of application. In addition, the specificity of the sensor has been compared with other viruses and sensing media, which is highly satisfactory due to the specific binding of the antibody on the sensor surface. The biosensor's sensitivity in clinically isolated Norovirus is compared with real-time quantitative reverse transcription-polymerase chain (RT-qPCR) and found to be satisfactory for its practical application. The sensor fabrication and detection mechanism are straightforward and rapid, giving it a significant advantage over other electrochemical methods for its possible up-gradation for kit development.