DEVELOPMENT OF AN IMPROVED METHOD FOR MEASURING NEUTRALIZING ANTIBODY TO ROTAVIRUS
DEVELOPMENT OF AN IMPROVED METHOD FOR MEASURING NEUTRALIZING ANTIBODY TO ROTAVIRUS
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DOI：
10.1016/0166-0934(91)90013-p
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发表时间：
1991-06-01
影响因子：
3.1
通讯作者：
WARD, RL
中科院分区：
文献类型：
--
作者：
KNOWLTON, DR;SPECTOR, DM;WARD, RL
开发了一种改进的测定法来测量针对轮状病毒的中和抗体滴度。 该程序使用与通过焦点还原中和（FRN）测定确定抗体滴度所执行的相同的初始步骤。 然而，不是在用荧光素染色后对受感染的细胞进行计数，而是通过使用 ELISA 对病毒抗原产生进行定量来确定中和抗体对病毒感染性的降低。 对于四种原型轮状病毒（代表血清型 1-4）中的每一种，在 ELISA 吸光度值和病灶形成单位之间发现了线性关系。 因此，中和 60% 感染性病毒的血清稀释度（即中和抗体滴度）可以很容易地从吸光度值确定。 通过该方法发现的滴度与 FRN 测定发现的滴度相似且可重复。 由于该方法不那么费力，并且结果是通过客观而非主观方法获得的，因此它代表了对 FRN 测定的改进。
An improved assay was developed for measuring neutralizing antibody titers against rotaviruses. This procedure used the same initial steps as performed to determine antibody titers by the focus reduction neutralization (FRN) assay. However, instead of counting infected cells after staining with fluorescein, reductions in virus infectivity by neutralizing antibody were determined by quantitation of viral antigen production using an ELISA. A linear relationship was found between ELISA absorbance values and focus forming units for each of four prototype rotaviruses, representative of serotypes 1-4. Thus, the serum dilution that resulted in neutralization of 60% of infectious virus (i.e. the neutralizing antibody titer) could be readily determined from absorbance values. Titers found by this method were similar to and as reproducible as those found by the FRN assay. Because this method is less laborious and the results are obtained by objective rather than subjective methods, it represents an improvement over the FRN assay.