Hydrogen peroxide concentrations in leaves under natural conditions
Hydrogen peroxide concentrations in leaves under natural conditions
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DOI：
10.1093/jxb/erl004
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发表时间：
2006-01-01
影响因子：
6.9
通讯作者：
Cheeseman, John M.
中科院分区：
文献类型：
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作者：
Cheeseman, John M.
虽然H2 O2已牵连在许多植物的环境反应，正常水平和变异性很差，叶组织浓度的估计跨越三个数量级以上，即使在类似条件下的单一物种。这里，在确定（i）提取物中的H2 O2随时间稳定，（ii）添加到提取物中的H2 O2被定量回收，以及（iii）H2 O2校准曲线不受提取物影响（或定量影响）之后，报告了自然条件下的叶组织H2 O2含量。广泛的适用性的协议和叶浓度的变化进行了证明，使用组织收集从几个栖息地与三个，更广泛的实验。第一部分是对红树林Rhizophora mangle L.的生理生化研究。最低的H2 O2水平出现在清晨和接近日落时，在中午和晚上都有较高的水平。其次，使用五个温带物种在春季，浓度进行了比较，在温暖，阳光明媚的日子和凉爽，阴天。光叶七叶树在温暖的日子浓度较高，常春藤，车前草，和Viola sorada Wilid。而大果钩虫之间无差异。最后，研究了CO2浓度升高和臭氧对大豆的影响。Pioneer 9313115在自由空气气体浓缩富集（FACE）条件下。这两种补充剂都导致H2 O2升高。总体而言，平均叶，中午，仲夏H2 O2浓度范围从0.67 μ mol（gFW）（-1）在红树林和3.6 μ mol（gFW）（-1）在A。光叶草在所有研究中，最大的物种内差异仅为2.5倍。
While H2O2 has been implicated in numerous plant environmental responses, normal levels and variabilities are poorly established, and estimates of leaf tissue concentrations span more than three orders of magnitude, even in a single species under similar conditions. Here, leaf tissue H2O2 contents under natural conditions are reported after determining (i) that H2O2 in extracts was stable with time, (ii) that H2O2 added to the extract was recovered quantitatively, and (iii) that the H2O2 calibration curve was unaffected (or quantifiably affected) by the extract. The broad applicability of the protocol and variability in leaf concentrations were demonstrated using tissue collected from several habitats in association with three, more extensive, experiments. The first involved nychthemeral studies of the mangrove, Rhizophora mangle L. Lowest H2O2 levels occurred in the early morning and near sunset, with higher levels both at midday and at night. Second, using five temperate species in Spring, concentrations were compared on a warm, sunny day and a cool, cloudy day. Higher concentrations were found on the warm day for Aesculus glabra Willd., Glechoma hederacea L., Plantago major L., and Viola sorada Wilid., while there were no differences in Ouercus macrocarpa Michx. Finally, the effects of elevated CO2 and ozone were examined in soybean, Glycine max L. Pioneer 9313115 under Free Air gas Concentration Enrichment (FACE) conditions. Both supplements led to elevated H2O2. Overall, mean leaf, midday, and mid-summer H2O2 concentrations ranged from 0.67 mu mol (gFW)(-1) in mangrove to 3.6 mu mol (gFW)(-1) in A. glabra Willd. Greatest within-species differences were only 2.5-fold in any of the studies.