Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
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DOI：
10.1086/600109
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发表时间：
2009-08-15
影响因子：
6.4
通讯作者：
Saravia, Nancy Gore
中科院分区：
文献类型：
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作者：
Figueroa, Roger Adrian;Lozano, Leyder Elena;Saravia, Nancy Gore
背景利什曼原虫（Viannia）是粘膜利什曼病的主要病因。粘膜疾病的自然史和发病机制是神秘的。粘膜组织的寄生虫学评价受到传统取样方法的侵入性的限制。我们评估了26例皮肤利什曼病患者和2例粘膜皮肤利什曼病患者粘膜中利什曼原虫的存在。鼻粘膜、扁桃体和结膜的拭子样本采用聚合酶链反应和Southern印迹杂交进行分析。2例皮肤粘膜利什曼病患者和26例皮肤利什曼病患者中的21例（81%）粘膜组织中存在利什曼原虫动基体小环DNA（kDNA）。从24例患者中的14例（58%）鼻粘膜、28例患者中的13例（46%）扁桃体和24例患者中的6例（25%）结膜的拭子样本中扩增kDNA。在巴拿马利什曼原虫、圭亚那利什曼原虫和巴西利什曼原虫引起的皮肤病患者黏膜中均检测到kDNA。在利什曼原虫（Viannia）感染患者中，粘膜组织中无症状存在寄生虫可能很常见。
Background. Leishmania (Viannia) species are the principal cause of mucosal leishmaniasis. The natural history and pathogenesis of mucosal disease are enigmatic. Parasitological evaluation of mucosal tissues has been constrained by the invasiveness of conventional sampling methods.Methods. We evaluated the presence of Leishmania in the mucosa of 26 patients with cutaneous leishmaniasis and 2 patients with mucocutaneous leishmaniasis. Swab samples of the nasal mucosa, tonsils, and conjunctiva were analyzed using polymerase chain reaction with LV-B1 primers and Southern blot hybridization.Results. Two patients with mucocutaneous leishmaniasis and 21 (81%) of 26 patients with cutaneous leishmaniasis had Leishmania kinetoplast minicircle DNA (kDNA) in mucosal tissues. kDNA was amplified from swab samples of nasal mucosa from 14 (58%) of 24 patients, tonsils from 13 (46%) of 28 patients, and conjunctiva from 6 (25%) of 24 patients. kDNA was detected in the mucosa of patients with cutaneous disease caused by Leishmania panamensis, Leishmania guyanensis, and Leishmania braziliensis.Conclusion. The asymptomatic presence of parasites in mucosal tissues may be common in patients with Leishmania (Viannia) infection.