The Fur-like protein Mur of Rhizobium leguminosarum is a Mn2+-responsive transcriptional regulator
The Fur-like protein Mur of Rhizobium leguminosarum is a Mn2+-responsive transcriptional regulator
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DOI：
10.1099/mic.0.26961-0
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发表时间：
2004-05-01
期刊：
影响因子：
2.8
通讯作者：
Johnston, AWB
中科院分区：
文献类型：
--
作者：
Díaz-Mireles, E;Wexler, M;Johnston, AWB
在野生型豆科根瘤菌中，SitABCD操纵子指定了一种Mn2+转运蛋白，在这种金属存在的情况下生长的细胞中，该转运蛋白的表达严重减少。豆类根瘤菌基因MUR(锰吸收调节因子)的突变，其产物类似于Fur转录调节因子，导致SitABCD在Mn2+存在时高水平表达。在凝胶迁移率分析中，纯化的豆类根霉Mur蛋白至少与SitABCD启动子区域附近的两个区域结合，尽管该DNA没有传统的共识毛皮结合序列(FUR盒)。因此，与毛皮结合Fe2+的伽马蛋白细菌不同，豆类根霉毛皮同源物Mur起到了Mn-2响应转录调节的作用。
In wild-type Rhizobium leguminosarum, the sitABCD operon specifies a Mn2+ transporter whose expression is severely reduced in cells grown in the presence of this metal. Mutations in the R. leguminosarum gene, mur (manganese uptake regulator), whose product resembles the Fur transcriptional regulator, cause high-level expression of sitABCD in the presence of Mn2+. In gel-shift mobility assays, purified R. leguminosarum Mur protein bound to at least two regions near the sitABCD promoter region, although this DNA has no conventional consensus Fur-binding sequences (fur boxes). Thus, in contrast to gamma-proteobacteria, where Fur binds Fe2+, the R. leguminosarum Fur homologue, Mur, act as a Mn-2-responsive transcriptional regulator.