Localization of connexin26 and connexin32 in putative CO2-chemosensitive brainstem regions in rat
Localization of connexin26 and connexin32 in putative CO2-chemosensitive brainstem regions in rat
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DOI：
10.1016/s0034-5687(01)00299-7
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发表时间：
2001-12-01
期刊：
影响因子：
--
通讯作者：
O'Neal, MH
中科院分区：
文献类型：
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作者：
Solomon, IC;Halat, TJ;O'Neal, MH
最近的研究表明，通过间隙连接发生的细胞间偶联可能在CO2化学接受中起作用。在这里，我们使用免疫印迹和免疫组织化学分析来研究缝隙连接蛋白连接蛋白26 （Cx26）和连接蛋白32 （Cx32）在新生大鼠和成年大鼠假定的二氧化碳化学敏感脑干区域的存在、分布和细胞定位。免疫印迹分析显示，两种Cx亚型都在假定的二氧化碳化学敏感脑干区域表达；然而，观察到区域表达差异。免疫组织化学实验证实Cx在每个假定的co2化学敏感脑干区域表达，并进一步证明Cx26和Cx32存在于神经元中，Cx26也存在于这些区域的星形胶质细胞中。因此，我们的研究结果表明，在新生大鼠和成年大鼠中，这些区域可能存在间隙连接通信。我们认为，间隙连接蛋白Cx26和Cx32，至少在一定程度上，构成了这种间隙连接通讯的神经解剖学底物，这被假设在中枢CO2化学接受中发挥作用。(C) 2001 Elsevier Science B.V.版权所有
Recent studies have suggested that cell-to-cell coupling, which occurs via gap junctions, may play a role in CO2 chemoreception. Here, we used immunoblot and immunohistochemical analyses to investigate the presence, distribution, and cellular localization of the gap junction proteins connexin-26 (Cx26) and connexin32 (Cx32) in putative CO2-chemosensitive brainstem regions in both neonatal and adult rats. Immunoblot analyses revealed that both Cx subtypes were expressed in putative CO2-chemosensitive brainstem regions; however, regional differences in expression were observed. Immunohistochemical experiments confirmed Cx expression in each of the putative CO2-chemosensitive brainstem regions, and further demonstrated that Cx26 and Cx32 were found in neurons and Cx26 was also found in astrocytes in these regions. Thus, our findings suggest the potential for gap junctional communication in these regions in both neonatal and adult rats. We propose that the gap junction proteins Cx26 and Cx32, at least in part, form the neuroanatomical substrate for this gap junctional communication, which is hypothesized to play a role in central CO2 chemoreception. (C) 2001 Elsevier Science B.V. All rights reserved.