Extracellular potassium regulates the chloride reversal potential in cultured hippocampal neurons
Extracellular potassium regulates the chloride reversal potential in cultured hippocampal neurons
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DOI：
10.1016/j.brainres.2008.02.038
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发表时间：
2008-04-18
期刊：
影响因子：
2.9
通讯作者：
Woodin, Melanie A.
中科院分区：
文献类型：
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作者：
Balena, Trevor;Acton, Brooke A.;Woodin, Melanie A.
哺乳动物海马中抑制性GABA能突触传递依赖于Cl-（E-Cl）的超极化逆转电位。为了研究E-Cl超极化的调节，我们在低浓度或高浓度的KCl（分别为2.6或18.7 mM）中培养海马神经元两周。然后使用双穿孔膜片钳技术从含有3 mM K的标准细胞外溶液记录神经元。低KCl培养的神经元激发自发动作电位（AP; 0.33 +/- 0.11 Hz），而高KCl培养的神经元处于静止状态，导致AP活性的显著差异（p=0.042）。这种高KCl诱导的活动减少伴随着AP阈值和AP阈值的去极化（p
Inhibitory GABAergic synaptic transmission in the mammalian hippocampus depends upon a hyperpolarized reversal potential for Cl- (E-Cl). To examine the regulation of E-Cl hyperpolarization we cultured hippocampal neurons for two weeks in either a low- or a high-concentration of KCl (2.6 or 18.7 mM, respectively). Neurons were then recorded from standard extracellular solution containing 3 mM K, using the dual perforated patch clamp technique. Low-KCl cultured neurons fired spontaneous action potentials (APs; 0.33 +/- 0.11 Hz), while high-KCl cultured neurons were quiescent, resulting in a significant difference in AP activity (p=0.042). This high-KCl-induced decrease in activity was accompanied by depolarizations of both the AP threshold (p