Preparation and evaluation of paclitaxel-imprinted polymers with a rosin-based crosslinker as the stationary phase in high-performance liquid chromatography
Preparation and evaluation of paclitaxel-imprinted polymers with a rosin-based crosslinker as the stationary phase in high-performance liquid chromatography
复制标题
以松香基交联剂为固定相的紫杉醇印迹聚合物的制备及高效液相色谱评价
DOI：
10.1016/j.chroma.2017.04.048
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发表时间：
2017-06-16
影响因子：
4.1
通讯作者：
Jiang, Jianxin
中科院分区：
文献类型：
--
作者：
Li, Pengfei;Wang, Ting;Jiang, Jianxin
采用微悬浮聚合法制备了紫杉醇（PTX）分子印迹聚合物（MIP）微球，并将其用作高效液相色谱（HPLC）固定相，用于紫杉醇及其结构类似物多西他赛（DOC）的分离。对于MIP的合成，乙二醇马来松香酸酯丙烯酸酯和2-乙烯基吡啶分别用作交联剂和功能单体。采用扫描电镜、激光粒度仪、氮吸附法和热重分析等方法对MIP微球进行了表征。结果表明，所制备的分子印迹聚合物具有均匀的孔径分布，且具有良好的热稳定性。将这些MIP微球填充到不锈钢柱中，用于选择性分离PTX和DOC。在最佳色谱条件下，分离因子为2.54，印迹因子为2.37。此外，从van 't霍夫图中得到的热力学数据显示，与空间互补作用相比，官能团相互作用对分离的贡献更大，且是双极性驱动的.微量热法研究了分析物在MIP表面的结合机制。(C)2017爱思唯尔B. V.保留所有权利。
In this study, molecularly imprinted polymer (MIP) microspheres for paclitaxel (PTX) were prepared by microsuspension polymerization and used as a stationary phase in high-performance liquid chromatography (HPLC) for the separation of PTX and its structural analog docetaxel (DOC). For MIP synthesis, ethylene glycol maleic rosinate acrylate and 2-vinylpyridine were used as the cross-linker and functional monomer, respectively. MIP microspheres were characterized by scanning electron microscopy, laser granulometry, nitrogen sorption porosimetry, and thermogravimetric analysis. Results indicated the formation of regular MIPs with an even pore size distribution; furthermore, these MIPs exhibited excellent thermal stability. These MIP microspheres were packed into a stainless steel column for the selective separation of PTX and DOC. Under optimum chromatographic conditions, a separation factor of 2.54 and an imprinting factor of 2.37 were obtained. In addition, thermodynamic data obtained from van't Hoff plots revealed enthalpy-driven separation and higher contribution from functional group interactions as compared with that from steric complementary interactions. Microcalorimetry was employed to investigate the binding mechanisms of the analytes on the MIP surface. (C) 2017 Elsevier B.V. All rights reserved.