Inhibition of creatine kinase activity by ethylene oxide.
Inhibition of creatine kinase activity by ethylene oxide.
复制标题
环氧乙烷抑制肌酸激酶活性。
DOI：
10.1136/oem.47.1.44
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
I. Tanaka
中科院分区：
文献类型：
--
作者：
M. Matsuoka;H. Igisu;N. Inoue;H. Hori;I. Tanaka
将老鼠暴露在500ppm的环氧乙烷中，每天6小时，每周3次，持续12周，血清肌酸激酶活性降低了40%以上。唯一的另一个变化是甘油三酯浓度略有下降。暴露四周后，大脑、脊髓和肌肉中的天冬氨酸氨基转移酶和乳酸脱氢酶活性都没有受到影响，但肌酸激酶活性明显受到抑制。在体外，环氧乙烷抑制了脑匀浆和纯化的肌肉酶制剂中的肌酸激酶活性。二硫苏糖醇不能抵消环氧乙烷的作用。环氧乙烷虽然使纯化的肌酸激酶中的巯基含量显著减少，但该酶仍表现出中等活性。因此，环氧乙烷在体内和体外都抑制肌酸激酶的活性，而且这种抑制似乎与酶中的巯基的破坏无关。
Exposure of rats to 500 ppm ethylene oxide for six hours a day, three times a week, for 12 weeks, lowered serum creatine kinase activity by more than 40%. The only other change was a slightly decreased triglyceride concentration. After four weeks of exposure, neither aspartate aminotransferase nor lactate dehydrogenase activity in brain, spinal cord, and muscle was affected but creatine kinase activity was clearly inhibited. In vitro, ethylene oxide inhibited creatine kinase activity in brain homogenate and in a purified muscle enzyme preparation. Dithiothreitol did not counteract the effect of ethylene oxide. Though the amount of sulphydryl groups in purified creatine kinase was decreased considerably by exposure to ethylene oxide, the enzyme still showed moderate activity. Thus ethylene oxide inhibits creatine kinase activity in vivo and in vitro and the inhibition appears to be unrelated to the disruption of sulphydryl groups in the enzyme.