Population genetic structure of Anopheles gambiae mosquitoes on Lake Victoria islands, west Kenya -: art. no. 48
Population genetic structure of Anopheles gambiae mosquitoes on Lake Victoria islands, west Kenya -: art. no. 48
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DOI：
10.1186/1475-2875-3-48
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发表时间：
2004-12-01
期刊：
影响因子：
3
通讯作者：
Yan, GY
中科院分区：
文献类型：
--
作者：
Chen, H;Minakawa, N;Yan, GY
背景：了解冈比亚岛按蚊种群的遗传结构对当前的蚊虫控制策略和利用转基因蚊子（GMM）提出的策略具有重要意义。岛屿上基因隔离的蚊子种群是检测GMM的潜在地点。本研究的目的是确定肯尼亚西部维多利亚湖岛屿上冈比亚拟南蝽种群的遗传结构。方法：利用6个微卫星标记，对维多利亚湖5个岛屿和周边大陆地区6个村庄的13个冈比亚古蠓种群的遗传多样性和种群遗传结构进行了分析。采样点的距离范围在2.5 ~ 35.1公里之间。结果：岛蚊种群与邻近大陆种群的遗传多样性水平相近。海岛人群平均等位基因数为7.3个，大陆人群平均等位基因数为6.8个。岛屿种群和大陆种群的平均杂合度分别为0.32和0.28。岛屿种群之间（F-ST = 0.019）和岛屿种群与大陆种群之间（F-ST = 0.003）存在较低但具有统计学意义的遗传结构。共发现12个私人等位基因，其中9个来自岛屿种群。结论：岛屿种群与大陆种群之间存在一定程度的遗传分化。岛屿和大陆蚊子种群之间的大量基因流动可能是风或人类辅助传播的结果。如果将维多利亚湖上的岛屿作为GMM释放计划的试验点，应大力监测岛屿之间以及岛屿与大陆之间的蚊子扩散情况。
Background: Understanding the genetic structure of island Anopheles gambiae populations is important for the current tactics in mosquito control and for the proposed strategy using genetically- modified mosquitoes ( GMM). Genetically- isolated mosquito populations on islands are a potential site for testing GMM. The objective of this study was to determine the genetic structure of A. gambiae populations on the islands in Lake Victoria, western Kenya.Methods: The genetic diversity and the population genetic structures of 13 A. gambiae populations from five islands on Lake Victoria and six villages from the surrounding mainland area in the Suba District were examined using six microsatellite markers. The distance range of sampling sites varied between 2.5 and 35.1 km.Results: A similar level of genetic diversity between island mosquito populations and adjacent mainland populations was found. The average number of alleles per locus was 7.3 for the island populations and 6.8 for the mainland populations. The average observed heterozygosity was 0.32 and 0.28 for the island and mainland populations, respectively. A low but statistically significant genetic structure was detected among the island populations ( F-ST = 0.019) and between the island and mainland populations (F-ST = 0.003). A total of 12 private alleles were found, and nine of them were from the island populations.Conclusion: A level of genetic differentiation between the island and mainland populations was found. Large extent of gene flow between the island and mainland mosquito populations may result from wind- or human- assisted dispersal. Should the islands on Lake Victoria be used as a trial site for the release program of GMM, mosquito dispersal between the islands and between the island and the mainland should be vigorously monitored.