THE HIGH MOBILITY GROUP PROTEIN HMG-I(Y) IS REQUIRED FOR NF-KAPPA-B-DEPENDENT VIRUS INDUCTION OF THE HUMAN IFN-BETA GENE
THE HIGH MOBILITY GROUP PROTEIN HMG-I(Y) IS REQUIRED FOR NF-KAPPA-B-DEPENDENT VIRUS INDUCTION OF THE HUMAN IFN-BETA GENE
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DOI：
10.1016/0092-8674(92)90554-p
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发表时间：
1992-11-27
期刊：
影响因子：
64.5
通讯作者：
MANIATIS, T
中科院分区：
文献类型：
--
作者：
THANOS, D;MANIATIS, T
在这篇论文中，我们发现NF-κ B和高迁移率族蛋白I（Y）（HMG I（Y））都是病毒诱导人干扰素-β（IFN-β）基因所必需的。NF-κ B通过在大沟中的接触结合到10 bp调节序列的末端区域，而HMG I（Y）通过在小沟中的接触识别相同序列的中心区域。干扰任一蛋白结合的突变降低病毒诱导水平，并且该基因的激活可以被NF-κ B或HMG I（Y）反义RNA阻断。HMG I（Y）刺激NF-κ B与IFN-β启动子的结合，并且它也可以作为NF-κ B转录活性的启动子特异性辅助因子。
In this paper, we show that both NF-kappaB and the high mobility group protein I(Y) (HMG I(Y)) are required for virus induction of the human interferon-beta (IFN-beta) gene. NF-kappaB binds to the terminal regions of a 10 bp regulatory sequence through contacts in the major groove, while HMG I(Y) recognizes the central region of the same sequence through contacts in the minor groove. Mutations that interfere with binding of either protein decrease the level of virus induction, and activation of the gene can be blocked by either NF-kappaB or HMG I(Y) antisense RNA. HMG I(Y) stimulates the binding of NF-kappaB to the IFN-beta promoter, and it may also function as a promoter-specific accessory factor for NF-kappaB transcriptional activity.