In vitro and in vivo anti-inflammatory activity of digested peptides derived from salmon myofibrillar protein conjugated with a small quantity of alginate oligosaccharide
In vitro and in vivo anti-inflammatory activity of digested peptides derived from salmon myofibrillar protein conjugated with a small quantity of alginate oligosaccharide
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DOI：
10.1080/09168451.2015.1031075
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发表时间：
2015-09-02
影响因子：
1.6
通讯作者：
Saeki, Hiroki
中科院分区：
文献类型：
--
作者：
Saigusa, Musashi;Nishizawa, Mizuho;Saeki, Hiroki
研究了鲑鱼肌原纤维蛋白（Mf）作为食用抗炎产品的来源。由胃蛋白酶和胰蛋白酶逐步消化Mf（不含碳水化合物）产生的肽对脂多糖刺激的RAW 264.7巨噬细胞分泌炎症相关化合物的影响很小。然而，在山梨醇存在下，通过Maillard反应将Mf与海藻酸寡糖（AO; 19 μ g/mg protein） （dMSA）偶联制备多肽，可显著降低促炎介质一氧化氮、肿瘤坏死因子(TNF)- α和白细胞介素(IL)-6的分泌，以及TNF- α、IL-6、诱导型一氧化氮合酶和环氧化酶-2的mRNA表达。此外，dMSA在卡拉胶诱导的小鼠足跖水肿模型中抑制急性炎症，但对自然杀伤细胞的细胞毒活性或巨噬细胞吞噬没有影响。这些结果提示，鱼蛋白偶联AO可能是一种具有抗炎功能的潜在食品材料。
Salmon myofibrillar protein (Mf) was investigated as a source of edible anti-inflammatory products. Peptides produced by stepwise digestion of Mf (without carbohydrate) with pepsin and trypsin had little effect on the secretion of inflammation-related compounds from lipopolysaccharide-stimulated RAW 264.7 macrophage cells. However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mu g/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Additionally, dMSA inhibited acute inflammation in a carrageenan-induced model of paw edema in mice, but had no effect on natural killer cell cytotoxic activity or macrophage phagocytosis. These results suggest that fish Mf conjugated with AO may be a potential food material with anti-inflammatory function.