ORIGIN OF VARIABILITY IN QUANTAL SIZE IN CULTURED HIPPOCAMPAL-NEURONS AND HIPPOCAMPAL SLICES
ORIGIN OF VARIABILITY IN QUANTAL SIZE IN CULTURED HIPPOCAMPAL-NEURONS AND HIPPOCAMPAL SLICES
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DOI：
10.1073/pnas.87.14.5359
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发表时间：
1990-07-01
影响因子：
11.1
通讯作者：
STEVENS, CF
中科院分区：
文献类型：
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作者：
BEKKERS, JM;RICHERSON, GB;STEVENS, CF
突触量子的大小已被发现在培养的海马神经元中显示出相当大的差异，但这种差异的来源此前尚不清楚。我们现在已经使用全细胞膜片钳记录比较了培养的海马神经元和海马片上局部诱发的微小兴奋性突触后电流的特性。在两种材料中，微小兴奋性突触后电流大小的变异性相似，当只刺激一个或几个突触突触时，培养的神经元发生变化。因此，微小兴奋性突触后电流幅度的变异性不是培养神经元的伪影，主要是由单个突触内的变异性引起的。讨论了这种可变性的可能来源。
The size of synaptic quanta has been found to display considerable variation in cultured hippocampal neurons, but the source of this variability was previously unknown. We have now compared the properties of locally evoked miniature excitatory postsynaptic currents in cultured hippocampal neurons and in thin hippocampal slices using whole-cell patch-clamp recordings. The variability in miniature excitatory postsynaptic currrent size was similar in both preparations and occurred in cultured neurons when only one or a few synaptic boutons were stimulated. Thus, the variability in miniature excitatory postsynaptic current amplitude is not an artifact of cultured neurons and arises predominantly from variability within a single bouton. Possible origins of this variability are discussed.