cDNA characterization and expression analysis of two arylphorin-like hexameric protein genes from the diamondback moth, Plutella xylostella (L.)
cDNA characterization and expression analysis of two arylphorin-like hexameric protein genes from the diamondback moth, Plutella xylostella (L.)
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DOI：
10.1002/arch.20168
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Tsumuki, Hisaaki
中科院分区：
文献类型：
--
作者：
Ashfaq, Muhammad;Sonoda, Shoji;Tsumuki, Hisaaki
我们克隆和表征了两个六聚体的存储蛋白基因，PxAry 1和PxAry 2，从小菜蛾，并研究了在不同的发育阶段，并在处理的保幼激素（JH）类似物的表达模式。PxAry 1和PxAry 2的完整编码序列分别由2，097和2，094 bp组成，分别具有699和698个氨基酸残基。在N-末端预测了16个氨基酸的信号肽。根据系统发育分析和氨基酸组成（芳香族氨基酸> 16%），PxAry 1和PxAry 2属于arylophorin样蛋白基因。利用北方杂交和RT-PCR分析表明，不同水平的基因表达的发育阶段，性别之间的差异很小。这两个基因在四龄幼虫的表达被抑制后，用JH-类似物处理。Southern杂交显示基因组中存在多个arylphorin基因。Arch.
We cloned and characterized two hexameric storage protein genes, PxAry1 and PxAry2, from Plutella xylostello and investigated the expression pattern in different developmental stages and in response to treatment by a juvenile hormone (JH) analog. The complete coding sequences of PxAry1 and PxAry2 are comprised of 2,097 and 2,094 bp with 699 and 698 amino acid residues, respectively. Signal peptides of 16 amino acids are predicted at the N-termini. According to both the phylogenetic analysis and amino acid composition (> 16% aromatic amino acids), PxAry1 and PxAry2 belong to the arylphorin-like protein genes. Analysis using Northern hybridization and RT-PCR showed varying levels of genes expression in the developmental stages with a small difference between sexes. Expression of both genes in fourth instar larvae was suppressed after treatment with a JH-analog. Southern hybridization revealed the presence of multiple arylphorin genes in the genome. Arch.