Mitochondrial Genomics Reveals Shared Phylogeographic Patterns and Demographic History among Three Periodical Cicada Species Groups
Mitochondrial Genomics Reveals Shared Phylogeographic Patterns and Demographic History among Three Periodical Cicada Species Groups
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DOI：
10.1093/molbev/msz051
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发表时间：
2019-06-01
影响因子：
10.7
通讯作者：
Li, Hu
中科院分区：
文献类型：
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作者：
Du, Zhenyong;Hasegawa, Hiroki;Li, Hu
全有丝分裂基因组（MG）测序的大规模应用对于解决部分有丝分裂基因组序列或核标记无法解决的复杂的地理分布模式具有巨大的潜力。北美周期性蝉（Magicicada）以其在北部和南部分别以17年和13年的间隔周期性大量出现而闻名。Magicicada包括三个物种组，每个组包含一个17年的物种和一个或两个13年的物种。在每个生命周期中，周期性蝉的单龄群体（称为窝）在不同年份出现，大多数窝包含所有三个物种组的成员。17年和13年的蝉分别有12窝和3窝。种群间的地理关系和种组内的育雏尚未明确解决。我们分析了125个完整的MG序列，从所有的育雏和7个物种在3个物种组，以确定地理和异时种群和它们的生活史的分歧历史。我们的有丝分裂基因组学遗传学分析清楚地表明，这三个物种组中的每一个都有很大程度上相似的地理分区（东部，中部和西部）和人口统计学历史（末次冰期后的快速人口扩张）。有丝分裂基因组的单系发生也部分地解决了育雏多样化的过程，这可以解释为假设的暂时的生命周期的转变，并表明，没有13和17岁的物种在物种组wasmonophytic，可能是由于它们之间的基因流。我们的研究结果清楚地揭示了三个Magicicada种组的地理结构，证明了全MG序列数据在地理研究中的优势。
The mass application of whole mitogenome (MG) sequencing has great potential for resolving complex phylogeographic patterns that cannot be resolved by partial mitogenomic sequences or nuclear markers. North American periodical cicadas (Magicicada) are well known for their periodical mass emergence at 17- and 13-year intervals in the north and south, respectively. Magicicada comprises three species groups, each containing one 17-year species and one or two 13-year species. Within each life cycle, single-aged cohorts, called broods, of periodical cicadas emerge in different years, and most broods contain members of all three species groups. There are 12 and three extant broods of 17-and 13-year cicadas, respectively. The phylogeographic relationships among the populations and broods within the species groups have not been clearly resolved. We analyzed 125 whole MG sequences from all broods and seven species within three species groups to ascertain the divergence history of the geographic and allochronic populations and their life cycles. Our mitogenomic phylogeny analysis clearly revealed that each of the three species groups had largely similar phylogeographic subdivisions (east, middle, and west) and demographic histories (rapid population expansion after the last glacial period). The mitogenomic phylogeny also partly resolved the brood diversification process, which could be explained by hypothetical temporary life cycle shifts, and showed that none of the 13-and 17-year species within the species groups wasmonophyletic, possibly due to gene flow between them. Our findings clearly reveal phylogeographic structures in the three Magicicada species groups, demonstrating the advantage of whole MG sequence data in phylogeographic studies.