Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels
Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels
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DOI：
10.1016/j.bbrc.2010.02.053
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发表时间：
2010-03-19
影响因子：
3.1
通讯作者：
Hara, Yukio
中科院分区：
文献类型：
--
作者：
Yamawaki, Hideyuki;Tsubaki, Naoya;Hara, Yukio
Omentin是最近发现的一种脂肪组织来源的细胞因子，与肥胖相关的心血管疾病有关。在本研究中，我们验证了网膜素可以直接影响离体血管的血管反应性的假设。在内皮完整的大鼠离体主动脉，网膜素（300 ng/ml，30分钟）预处理抑制去甲肾上腺素（NA; 1 nM-1 μ M）诱导的浓度依赖性收缩。在NA（100 nM）预收缩的主动脉中，网膜素（1-300 ng/ml）直接诱导内皮依赖性舒张。一氧化氮（NO）合酶（NOS）抑制剂N-G-硝基-L-精氨酸甲酯（100 μ M，30分钟）抑制松弛，而PI 3 K/Akt抑制剂LY 294002（10 μ M，30分钟）或酪氨酸激酶抑制剂染料木黄酮（30 μ M，30分钟）无效。Omentin（300 ng/ml，5 min）诱导内皮NOS在丝氨酸1177处的磷酸化，但不诱导Akt在丝氨酸473处的磷酸化。网膜素（1-300 ng/ml）也能舒张NA预收缩的肠系膜动脉。本研究首次证实网膜素对离体血管具有舒张作用，其作用是通过内皮源性NO介导的。（C）2010 Elsevier Inc. All rights reserved.
Omentin is a recently identified adipose tissue-derived cytokine and is implicated in obesity-related cardiovascular disorders. In the present study, we tested the hypothesis that omentin could directly affect vascular reactivity of isolated blood vessels. In endothelium-intact rat isolated aorta, pretreatment with omentin (300 ng/ml, 30 min) inhibited noradrenaline (NA; 1 nM-1 mu M)-induced concentration-dependent contraction. In NA (100 nM)-pre-contracted aorta, omentin (1-300 ng/ml) directly induced an endothelium-dependent relaxation. While a nitric oxide (NO) synthase (NOS) inhibitor, N-G-nitro-L-arginine methyl ester (100 mu M, 30 min) inhibited the relaxation, a PI3 K/Akt inhibitor, LY294002 (10 mu M, 30 min) or a tyrosine kinase inhibitor, genistein (30 mu M, 30 min) was ineffective. Omentin (300 ng/ml, 5 min) induced a phosphorylation of endothelial NOS at serine 1177 but not a phosphorylation of Akt at serine 473. Omentin (1-300 ng/ml) also relaxed NA pre-contracted mesenteric artery. Present study for the first time demonstrated that omentin has a vasodilating effect on isolated blood vessels, which is mediated through endothelium-derived NO. (C) 2010 Elsevier Inc. All rights reserved.