CELLULAR-LOCALIZATION AND HORMONAL-REGULATION OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE RECEPTOR MESSENGER-RNAS IN THE RAT OVARY
CELLULAR-LOCALIZATION AND HORMONAL-REGULATION OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE RECEPTOR MESSENGER-RNAS IN THE RAT OVARY
复制标题
DOI：
10.1210/mend-5-10-1405
复制
发表时间：
1991-10-01
影响因子：
--
通讯作者：
MAYO, KE
中科院分区：
文献类型：
--
作者：
CAMP, TA;RAHAL, JO;MAYO, KE
垂体促性腺激素FSH和LH是调节卵巢和睾丸中配子发生和类固醇生成的关键激素。介导这些激素的生物活性的细胞表面受体被认为在卵巢中以细胞特异性方式表达，并随着动物在生殖周期中的进展而受到调节。利用克隆的受体cDNA，我们研究了大鼠卵巢FSH和LH受体mRNA的表达和激素调节。定量逆转录-聚合酶链反应扩增方案用于测量FSH和LH受体mRNA的相对水平，而原位杂交用于定位FSH和LH受体转录本。在未成熟动物中，在小卵泡的颗粒细胞中观察到低水平的FSH受体mRNA，而在这些相同卵泡的卵泡膜细胞中发现低水平的LH受体mRNA。PMSG刺激后，两种mRNA的水平增加，LH受体mRNA定位于大卵泡的颗粒细胞和卵泡膜细胞。用hCG进一步处理PMSG致敏动物导致下调，特别是颗粒细胞中LH受体mRNA的下调。在成年动物中，LH受体mRNA水平在发情周期中发生显著变化，特别是在排卵前LH峰之后。FSH受体mRNA水平显示出类似的变化模式，但FSH受体mRNA丰度较低，并且不像LH受体mRNA那样受到高度调节。FSH受体mRNA主要定位于健康发育卵泡的颗粒细胞，而LH受体mRNA在发情早晨主要定位于小卵泡的卵泡膜细胞，然后在非发情早晨出现在生长卵泡的颗粒细胞。LH受体mRNA在整个发情周期的大部分时间内也存在于间质组织和黄体中。我们的研究结果表明，促性腺激素受体基因的调控在一个复杂的方式在招聘，成熟和排卵的卵泡。
The pituitary gonadotropins FSH and LH are key hormones for regulating gametogenesis and steroidogenesis in the ovary and testis. The cell surface receptors that mediate the biological activities of these hormones are thought to be expressed in a cell-specific fashion in the ovary and are regulated as animals progress through the reproductive cycle. Using cloned receptor cDNAs, we have examined the expression and hormonal regulation of the ovarian FSH and LH receptor mRNAs in the rat. A quantitative reverse transcription-polymerase chain reaction amplification scheme was used to measure relative levels of the FSH and LH receptor mRNAs, while in situ hybridization was used to localize FSH and LH receptor transcripts. In immature animals, low levels of FSH receptor mRNA are observed in the granulosa cells of small follicles, while low levels of LH receptor mRNA are found in the thecal cells of these same follicles. After stimulation with PMSG, levels of both mRNAs increase, and the LH receptor mRNA is localized in both the granulosa and thecal cells of large follicles. Further treatment of PMSG-primed animals with hCG results in down-regulation, particularly of the LH receptor mRNA in granulosa cells. In adult animals, LH receptor mRNA levels change dramatically during the estrous cycle, particularly after the preovulatory LH surge. FSH receptor mRNA levels show a similar pattern of change, but the FSH receptor mRNA is of lower abundance and is not as highly regulated as the LH receptor mRNA. FSH receptor mRNA is confined to the granulosa cells of healthy developing follicles, whereas LH receptor mRNA is localized predominantly to thecal cells of small follicles on estrous morning, then appears in the granulosa cells of growing follicles by diestrous morning. LH receptor mRNA is also found in interstitial tissues and corpora lutea throughout much of the estrous cycle. Our results indicate that the gonadotropin receptor genes are regulated in a complex fashion during the recruitment, maturation, and ovulation of the ovarian follicle.