A systems biology approach for diagnostic and vaccine antigen discovery in tropical infectious diseases.
A systems biology approach for diagnostic and vaccine antigen discovery in tropical infectious diseases.
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DOI：
10.1097/qco.0000000000000193
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发表时间：
2015-10
影响因子：
3.9
通讯作者：
Felgner PL
中科院分区：
文献类型：
--
作者：
Liang L;Felgner PL
审查目的：需要改进诊断，更快地评估新出现或再次出现的传染病的存在、流行和传播情况。病原体检测策略的一种方法是基于分析宿主对感染的免疫反应。本文主要介绍了蛋白质微阵列技术的研究进展。最新发现：在这里，我们采用蛋白质微阵列方法来描述对多种感染性病原体的体液免疫反应，并确定与每种疾病相关的完整抗体谱系。这些研究的结果导致了诊断标记和潜在的亚单位疫苗候选的确定。这些来自30多种不同生物的结果也可以提供体液免疫反应的共同趋势的信息。摘要：利用蛋白质芯片鉴定与传染病挑战相关的抗体库的系统生物学方法已成为鉴定诊断标志物和潜在的亚单位疫苗候选者的有效方法，而且在提供血清反应性和血清诊断抗原的蛋白质组特征(功能和物理特性)方面也是一种强有力的方法。将病原体的检测与宿主免疫反应的全面评估相结合，将对特定病原体、宿主反应和疾病的临床表现之间的相关性提供新的理解。
Purpose of review: There is a need for improved diagnosis and for more rapidly assessing the presence, prevalence and spread of newly emerging or re-emerging infectious diseases. An approach to the pathogen-detection strategy is based on analyzing host immune response to the infection. This review focuses on a protein microarray approach for this purpose. Recent findings: Here we take a protein microarray approach to profile the humoral immune response to numerous infectious agents, and to identify the complete antibody repertoire associated with each disease. The results of these studies lead to the identification of diagnostic markers and potential subunit vaccine candidates. These results from over 30 different organisms can also provide information about common trends in the humoral immune response. Summary: Systems biology approach to identify the antibody repertoire associated with infectious diseases challenge using protein microarray has become a powerful method in identifying diagnostic markers and potential subunit vaccine candidates, and moreover, in providing information on proteomic feature (functional and physically properties) of seroreactive and serodiagnostic antigens. Combining the detection of the pathogen with a comprehensive assessment of the host immune response will provide a new understanding of the correlations between specific causative agents, the host response, and the clinical manifestations of the disease.