Estimating human ovarian non-growing follicle number: the application of modern stereology techniques to an old problem
Estimating human ovarian non-growing follicle number: the application of modern stereology techniques to an old problem
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DOI：
10.1093/humrep/dem137
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发表时间：
2007-08-01
影响因子：
6.1
通讯作者：
Klein, Nancy A.
中科院分区：
文献类型：
--
作者：
Charleston, Jay S.;Hansen, Karl R.;Klein, Nancy A.
背景技术背景：以前发表的关于人类卵巢中非生长卵泡（NGF）数量的报告采用了基于模型的方法进行数量估计。这些方法是时间密集型的，并且需要校正因子和假设，这最终限制了它们的准确性。在这里，我们描述了一个现代的分馏器/光学disector技术的修改，应用和验证的估计人类卵巢神经生长因子number.METHODS：48对正常的人类卵巢收集妇女（年龄8-51岁）进行选择性双侧卵巢切除术，器官捐赠，或尸检。在大体病理学检查后，利用系统随机取样获得组织，通过分馏器/光学分离器方法进行分析。通过计算观察到的误差系数（OCE）确定单个NGF计数的精密度。观察者内的变异性和一对卵巢之间的神经生长因子数量的变化也determined.RESULTS：平均OCE为16.6%，在较低的卵泡计数观察到较大的变化。在相同卵巢的重新计数实验中，NGF数量估计值的变化为15- 29%，除了在非常低的卵泡计数时变化更大，但绝对差异很小。有一对卵巢之间的NGF数量没有显着差异（Wilcoxon符号秩检验，P = 0.81）。结论：现代体视学方法提供了一个公正的，有效的方法估计人类卵巢中的NGF数量。一对卵巢中的两个卵巢含有相似数量的神经生长因子。
BACKGROUND: Previous published reports on the number of non-growing follicles (NGFs) in the human ovary have employed model-based methods for number estimates. These methods are time-intensive, and require correction factors and assumptions that ultimately limit their accuracy. Here, we describe the modification, application and validation of a modern fractionator/optical disector technique for the estimation of human ovarian NGF number.METHODS: Forty-eight pairs of normal human ovaries were collected from women (age 8-51 years) undergoing elective bilateral oophorectomy, organ donation, or from autopsy. After gross pathologic examination, systematic random sampling was utilized to obtain tissue for analysis by the fractionator/optical disector method. The precision of individual NGF counts was determined by calculating the observed coefficient of error (OCE). Intra-observer variability and variation in NGF number between ovaries within a pair were also determined.RESULTS: The mean OCE was 16.6% with larger variations observed at lower follicle counts. In recount experiments of the same ovary, NGF number estimates varied by 15-29%, except at very low follicle counts where variation was greater, but absolute differences were small. There was no significant difference in NGF number between ovaries within a pair (Wilcoxon signed rank test, P = 0.81).CONCLUSIONS: Modern stereology methods provide an unbiased, efficient method for estimating NGF number in the human ovary. Both ovaries within a pair contain similar numbers of NGFs.