Human hair growth in vitro: a model for the study of hair follicle biology.
Human hair growth in vitro: a model for the study of hair follicle biology.
复制标题
DOI：
10.1016/0923-1811(94)90036-1
复制
发表时间：
1994-07-01
影响因子：
4.6
通讯作者：
Kealey, T
中科院分区：
文献类型：
--
作者：
Philpott, M P;Sanders, D;Kealey, T
调节毛囊生长的因素仍然知之甚少。体外模型可能有助于阐明毛囊生物学的某些方面。我们开发了一种体外人类毛发生长模型，使我们能够将分离的人类毛囊维持长达10天，在此期间，它们继续以体内速率生长，产生角化的毛发纤维。我们已经表明，表皮生长因子（EGF）在我们的系统模仿EGF在绵羊体内脱毛作用，并建议，这是由于EGF刺激外根鞘（ORS）细胞增殖，导致在毛囊中的细胞-细胞相互作用的正常机制的破坏。我们确定转化生长因子-β（TGF-β）作为一种可能的毛囊生长的负调节因子，并表明生理水平的胰岛素样生长因子-I（IGF-I）可以支持与超生理水平的胰岛素相同的毛囊生长速率。此外，在缺乏胰岛素的情况下，毛囊显示过早进入退化样状态。这是通过IGF-I的生理水平来预防的。最后，我们证明了毛囊是一个有氧糖酵解，分解氨基的组织，并讨论了这种代谢的可能影响。
The factors that regulate hair follicle growth are still poorly understood. In vitro models may be useful in elucidating some aspects of hair follicle biology. We have developed an in vitro human hair growth model that enables us to maintain isolated human hair follicles for up to 10 days, during which time they continue to grow at an in vivo rate producing a keratinised hair fibre. We have shown that epidermal growth factor (EGF) in our system mimics the in vivo depilatory action of EGF in sheep, and suggest that this occurs as a result of EGF stimulating outer root sheath (ORS) cell proliferation which results in the disruption of normal mechanisms of cell-cell interaction in the hair follicle. We identify transforming growth factor-beta (TGF-beta) as a possible negative regulator of hair follicle growth and show that physiological levels of insulin-like growth factor-I (IGF-I) can support the same rates of hair follicle growth as supraphysiological levels of insulin. Furthermore, in the absence of insulin hair follicles show premature entry into a catagen-like state. This is prevented by physiological levels of IGF-I. Finally we demonstrate that the hair follicle is an aerobic glycolytic, glutaminolytic tissue and discuss the possible implications of this metabolism.