Candidate placental biomarkers for intrauterine alcohol exposure.
Candidate placental biomarkers for intrauterine alcohol exposure.
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DOI：
10.1111/j.1530-0277.2010.01373.x
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Redei EE
中科院分区：
文献类型：
--
作者：
Shukla PK;Sittig LJ;Ullmann TM;Redei EE
Fetal alcohol spectrum disorder (FASD) is a leading cause of non-genetic mental retardation and other neurodevelopmental deficits. Earlier diagnosis of FASD would greatly improve prognosis for individuals and families affected by this disorder. Here we identify candidate placental biomarkers in an animal model of FASD that recapitulates many aspects of human FASD. Pregnant Sprague-Dawley (SD) females were assigned to one of three diet groups on gestation day 8 (G8): Ethanol (E), Pair-fed (PF) or Control (C). E dams received ethanol-containing liquid diet and PF dams received isocaloric liquid diet in an amount that matched the paired E dam’s diet consumption the previous day. Control dams received lab chow and water ad libitum. Whole placentae from individual fetuses were collected on gestational day 21 (G21) for analyses. Western blotting and quantitative real-time RT-PCR were used to measure protein and mRNA levels of placental iodothyronine deiodinase III (Dio3), thyroid hormone receptor α1 (TRα1), and glucocorticoid receptor (GR). Placental mRNA levels of insulin-like growth factor 2 (Igf-2), pleckstrin homology-like domain family A member 2 (Phlda2), and cyclin-dependent kinase inhibitor 1C (Cdkn1c) were also measured. Placental protein and mRNA levels from ethanol (E)-consuming dams showed the following changes: increased Dio3, decreased TRα1 and decreased GR compared to both C and PF dams. Placental mRNA levels of intrauterine growth restriction (IUGR) markers Igf-2, Phlda2 and Cdkn1c were altered similarly in PF and E dams. We propose the specific pattern of increased Dio3 and decreased TRα1 and GR protein levels in the placenta as selective biomarker for intrauterine alcohol exposure.
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影响因子：
3.8
作者：
Gatford, K. L.;Simmons, R. A.;De Blasio, M. J.;Robinson, J. S.;Owens, J. A.
通讯作者：
Owens, J. A.
DOI：
10.1111/j.1530-0277.2009.01106.x
发表时间：
2010-03-01
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Gundogan F;Elwood G;Mark P;Feijoo A;Longato L;Tong M;de la Monte SM
通讯作者：
de la Monte SM
影响因子：
3.6
作者：
BEARER, CF;GOULD, S;COOK, CS
通讯作者：
COOK, CS
影响因子：
3
作者：
Mesquita, Ana Raquel;Wegerich, Yvonne;Almeida, Osborne F. X.
通讯作者：
Almeida, Osborne F. X.
DOI：
10.1111/j.1530-0277.2001.tb02295.x
发表时间：
2001-06-01
影响因子：
3.2
作者：
Glavas, MM;Hofmann, CE;Weinberg, J
通讯作者：
Weinberg, J