IMMUNOHISTOLOGICAL DEMONSTRATION OF PYRUVATE-KINASE ISOENZYME TYPE-L IN RAT WITH MONOCLONAL-ANTIBODIES
IMMUNOHISTOLOGICAL DEMONSTRATION OF PYRUVATE-KINASE ISOENZYME TYPE-L IN RAT WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1177/40.5.1374093
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发表时间：
1992-05-01
影响因子：
3.2
通讯作者：
REINACHER, M
中科院分区：
文献类型：
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作者：
DOMINGO, M;EINIG, C;REINACHER, M
制备并鉴定了4株抗大鼠丙酮酸激酶L同工酶(L-PK)的单抗。它们检测到至少两个不同的同工酶表位，如干扰结合分析和肽图绘制后的Western印迹分析所示。免疫组织化学方法检测石蜡包埋正常大鼠组织中L-PK同工酶的表达。L-PK仅见于肝细胞、肾近端小管、胰岛细胞和小肠绒毛上皮细胞。门静脉周围区肝细胞L-PK含量往往低于门静脉周围区。在肾脏中，L-PK的含量和分布在雄性和雌性大鼠之间存在明显差异。雄性动物肾皮质中L-PK活性高于雌性动物。男性肾脏内皮质带(直形近端小管)的L-PK含量高于外皮质带(卷曲的近端小管)。相比之下，雌性大鼠肾脏内侧皮质区的L-PK活性低于外侧皮质区。它们中只有一些在肾脏近端小管的两个部分显示了相同数量的同工酶。
Four monoclonal antibodies (MAb) specific for the L-type isoenzyme of rat pyruvate kinase (L-PK) were produced and characterized. They detect at least two different epitopes of the isoenzyme, as shown in interference binding assay and Western blot analysis after peptide mapping. The MAb were used in immunohistology to demonstrate the L-PK isoenzyme in paraffin-embedded normal rat tissues. L-PK was found only in hepatocytes, kidney proximal tubules, islet cells of pancreas, and epithelial cells of the villi of small intestine. The content of L-PK in hepatocytes was often lower in the periportal areas as compared with the periveneous zone. In kidneys a clearcut difference in L-PK content and distribution existed between male and female rats. Male animals possessed more L-PK in the kidney cortex than females. The L-PK content in the inner cortical zone (straight proximal tubules) was higher than in the outer cortical zone (convoluted proximal tubules) in kidneys of males. In contrast, female rats displayed less L-PK in the inner than in the outer cortical zone of the kidneys. Only some of them exhibited the same amount of the isoenzyme in both parts of the kidney proximal tubules.