Cold stress protein RBM3 responds to temperature change in an ultra-sensitive manner in young neurons.
Cold stress protein RBM3 responds to temperature change in an ultra-sensitive manner in young neurons.
复制标题
冷应激蛋白RBM3在年轻神经元中以超敏感的方式对温度的变化做出反应。
DOI：
10.1016/j.neuroscience.2015.08.012
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发表时间：
2015-10-01
期刊：
影响因子：
3.3
通讯作者：
Kochanek PM
中科院分区：
文献类型：
--
作者：
Jackson TC;Manole MD;Kotermanski SE;Jackson EK;Clark RS;Kochanek PM
临床上认为，极低体温至36.0°C与37.0°C并无显著差异。然而，36.0°C有可能刺激高度敏感的低温信号机制(S)并改变生物化学。就我们所知，还没有这样的超敏感途径/机制被描述。在这里，我们表明，与37℃对照相比，在33℃或36℃保持24或48小时的神经元和星形胶质细胞培养中，冷应激蛋白RNA结合基序3(RBM3)增加。在36℃培养的神经元也增加了全球蛋白质合成(GPS)。最后，我们发现褪黑素或成纤维细胞生长因子21(FGF21)可以促进降温到36℃的年轻神经元RBM3的上调。我们的结果表明，温度降低1℃可以通过上调神经元中的GPS来诱导多效性生化变化，这一过程可能是由RBM3介导的，这一过程可以被褪黑素或FGF21药理模拟和增强。
Extremely mild hypothermia to 36.0°C is not thought to appreciably differ clinically from 37.0°C. However, it is possible that 36.0°C stimulates highly sensitive hypothermic signaling mechanism(s) and alters biochemistry. To the best of our knowledge, no such ultra-sensitive pathway/mechanisms have been described. Here we show that cold stress protein RNA Binding motif 3 (RBM3) increases in neuron and astrocyte cultures maintained at 33°C or 36°C for 24 or 48h, compared to 37°C controls. Neurons cultured at 36°C also had increased global protein synthesis (GPS). Finally, we found that melatonin or fibroblast growth factor 21 (FGF21) augmented RBM3 upregulation in young neurons cooled to 36°C. Our results show that a 1°C reduction in temperature can induce pleiotropic biochemical changes by upregulating GPS in neurons which may be mediated by RBM3 and that this process can be pharmacologically mimicked and enhanced with melatonin or FGF21.