THE DEVELOPMENTAL RELATIONSHIPS OF THE NEURAL-TUBE AND THE NOTOCHORD - SHORT AND LONG-TERM EFFECTS OF THE NOTOCHORD ON THE DORSAL SPINAL-CORD
THE DEVELOPMENTAL RELATIONSHIPS OF THE NEURAL-TUBE AND THE NOTOCHORD - SHORT AND LONG-TERM EFFECTS OF THE NOTOCHORD ON THE DORSAL SPINAL-CORD
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DOI：
10.1016/0925-4773(95)00426-2
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发表时间：
1995-10-01
影响因子：
2.6
通讯作者：
LEDOUARIN, NM
中科院分区：
文献类型：
--
作者：
MONSOROBURQ, AH;BONTOUX, M;LEDOUARIN, NM
神经管腹侧半部的图案是脊索和底板的感应影响的结果。我们在这里研究了异位移植的脊索对神经管最后部，即顶骨和翼板发育的影响。我们发现，在它们的早期阶段，背侧基因被脊索背侧移植物抑制，正如先前在其他研究中所显示的那样。我们还发现，当脊索被植入顶板顶部的背侧位置时(而不是像以前在其他研究中所做的那样是横向的)，底板的基因特异性不会被诱导，运动神经元也不会分化。脊索阻止从顶板细胞形成内侧隔，并在E5和E7之间诱导它们的活跃增殖。顶板和背侧翼板来源的细胞从E7开始死亡，留下背侧截断的脊髓。如果脊索与矢状面成20度-30度，就会诱导出腹侧基因和结构，顶板就能正常分化。我们得出结论，屋顶板细胞对脊索信号表现出一种特殊的反应，其短程效应由此被显著地证明。
Patterning of the ventral half of the neural tube results from the inductive influence of the notochord and of the floor plate. We have studied here the effect of an ectopically grafted notochord on the development of the dorsalmost part of the neural tube i.e. roof prate and alar plates. We show that at their early stages, dorsal genes are repressed by the dorsal graft of a notochord, as shown previously in other studies. We found also that when the notochord is implanted in a mediodorsal position on top of the roof plate (and not laterally as previously performed in other studies) the genes specifics of the floor plate are not induced, and motoneurons do not differentiate. The notochord prevents the formation of the medial septum from roof plate cells and induces their active proliferation between E5 and E7. Roof and dorsal alar plates derived cells start to die from E7 onward leaving a dorsally truncated spinal cord. If the notochord is grafted at 20 degrees-30 degrees from the sagittal plane ventral genes and structures are induced and the roof plate differentiates normally. We conclude that roof plate cells exhibit a specific response to notochord signals, the short range effect of which is thus strikingly demonstrated.