DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China.
DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China.
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中国樟科植物DNA条形码评估及其系统发育关系的意义
DOI：
10.1371/journal.pone.0175788
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Liu ZF;Ci XQ;Li L;Li HW;Conran JG;Li J
樟科植物是热带和亚热带森林的重要组成部分，具有重要的生态和经济意义。由于缺乏明确的属和种之间的形态差异，该科是一个理想的情况下，测试的DNA条形码在种和属的鉴定和区分的效力。本研究对中国12属133种植物的matK、rbcL、trnH-psbA、ITS 2和ITS区序列进行了分析。我们测试了DNA条形码区分物种的能力，并作为纠正物种错误识别的替代工具。我们还使用rbcL+matK+trnH-psbA+ITS基因座来研究物种的系统发育关系。在基因区及其组合中，ITS区对种和属的识别效率最高，分别为57.5%和70%。DNA条形码对纠正物种错误识别也有积极作用（10.8%）。系统发育分析结果表明，中国樟科植物形成了3个单系分支，其中厚壳桂属（Cryptocarya group）是一个强有力的单系分支（PP = 1.00，BS = 100%）和包括波斯组、月桂属和肉桂属的分支也得到了强有力的支持（PP = 1.00，BS = 98%），而Caryodaphnopsis-Neocinnamomum仅获得中等支持（PP = 1.00，BS = 85%）。这项研究表明，分子条形码可以帮助筛选难以识别的家庭，如樟科，检测错误的物种鉴定，以及帮助重建系统发育关系。因此，DNA条形码可以通过提高准确性以及减少与物种识别相关的时间和成本来帮助大规模生物多样性库存和稀有物种保护。
Lauraceae are an important component of tropical and subtropical forests and have major ecological and economic significance. Owing to lack of clear-cut morphological differences between genera and species, this family is an ideal case for testing the efficacy of DNA barcoding in the identification and discrimination of species and genera. In this study, we evaluated five widely recommended plant DNA barcode loci matK, rbcL, trnH–psbA, ITS2 and the entire ITS region for 409 individuals representing 133 species, 12 genera from China. We tested the ability of DNA barcoding to distinguish species and as an alternative tool for correcting species misidentification. We also used the rbcL+matK+trnH–psbA+ITS loci to investigate the phylogenetic relationships of the species examined. Among the gene regions and their combinations, ITS was the most efficient for identifying species (57.5%) and genera (70%). DNA barcoding also had a positive role for correcting species misidentification (10.8%). Furthermore, based on the results of the phylogenetic analyses, Chinese Lauraceae species formed three supported monophyletic clades, with the Cryptocarya group strongly supported (PP = 1.00, BS = 100%) and the clade including the Persea group, Laureae and Cinnamomum also receiving strong support (PP = 1.00, BS = 98%), whereas the Caryodaphnopsis–Neocinnamomum received only moderate support (PP = 1.00 and BS = 85%). This study indicates that molecular barcoding can assist in screening difficult to identify families like Lauraceae, detecting errors of species identification, as well as helping to reconstruct phylogenetic relationships. DNA barcoding can thus help with large-scale biodiversity inventories and rare species conservation by improving accuracy, as well as reducing time and costs associated with species identification.