Archaeal ammonia oxidizers and nirS-type denitrifiers dominate sediment nitrifying and denitrifying populations in a subtropical macrotidal estuary
Archaeal ammonia oxidizers and nirS-type denitrifiers dominate sediment nitrifying and denitrifying populations in a subtropical macrotidal estuary
复制标题
DOI：
10.1038/ismej.2009.105
复制
发表时间：
2010-02-01
期刊：
影响因子：
11
通讯作者：
Robert, Stanley S.
中科院分区：
文献类型：
--
作者：
Abell, Guy C. J.;Revill, Andrew T.;Robert, Stanley S.
硝化和反硝化是氮循环的关键步骤。这些过程的耦合，影响氮在生态系统中的流动，需要硝化和反硝化微生物，在空间和时间上的密切相互作用。影响这些过程的微生物群落的多样性，时间和空间变化进行了研究，在氮循环，在菲茨罗伊河口，这是一个浑浊的亚热带河口在中部昆士兰州的12个站点。河口是排入大堡礁近岸区的营养物质的主要来源。氮通量的测量表明，在所有采样月份活跃的反硝化群落。微生物氨单加氧酶（AOA的amoA，硝化作用的功能标记）比β-AOB的amoA显著更丰富。亚硝酸还原酶基因，反硝化作用的功能标记，占主导地位的nirS，而不是nirK类型在所有网站在这一年。AOA社区占主导地位的Crenarchaeota的土壤/沉积物集群，在河口沉积物，海洋和陆地环境中发现的序列，而nirS序列显着更多样化（其中操作分类单位被定义为阈值的5%和15%的序列相似性），并密切相关的序列起源于河口沉积物。末端限制性片段长度多态性（T-RFLP）分析表明，AOA人口组成的空间变化沿着河口，而nirS人口的时间变化。个体T-RF优势度的统计分析表明，盐度和C：N与AOA的群落演替相关，而nirS型藻类群落与盐度和叶绿素α相关。The ISME Journal（2010）4，286-300; doi：10.1038/ismej.2009.105; 2009年10月1日在线发表
Nitrification and denitrification are key steps in nitrogen (N) cycling. The coupling of these processes, which affects the flow of N in ecosystems, requires close interaction of nitrifying and denitrifying microorganisms, both spatially and temporally. The diversity, temporal and spatial variations in the microbial communities affecting these processes was examined, in relation to N cycling, across 12 sites in the Fitzroy river estuary, which is a turbid subtropical estuary in central Queensland. The estuary is a major source of nutrients discharged to the Great Barrier Reef near-shore zone. Measurement of nitrogen fluxes showed an active denitrifying community during all sampling months. Archaeal ammonia monooxygenase (amoA of AOA, functional marker for nitrification) was significantly more abundant than Betaproteobacterial (beta-AOB) amoA. Nitrite reductase genes, functional markers for denitrification, were dominated by nirS and not nirK types at all sites during the year. AOA communities were dominated by the soil/sediment cluster of Crenarchaeota, with sequences found in estuarine sediment, marine and terrestrial environments, whereas nirS sequences were significantly more diverse (where operational taxonomic units were defined at both the threshold of 5% and 15% sequence similarity) and were closely related to sequences originating from estuarine sediments. Terminal-restriction fragment length polymorphism (T-RFLP) analysis revealed that AOA population compositions varied spatially along the estuary, whereas nirS populations changed temporally. Statistical analysis of individual T-RF dominance suggested that salinity and C:N were associated with the community succession of AOA, whereas the nirS-type denitrifier communities were related to salinity and chlorophyll-alpha in the Fitzroy river estuary. The ISME Journal (2010) 4, 286-300; doi:10.1038/ismej.2009.105; published online 1 October 2009