Mapping of the chromosome 17 BMD QTL in the F2 male mice of MRL/MpJ x SJL/J
Mapping of the chromosome 17 BMD QTL in the F2 male mice of MRL/MpJ x SJL/J
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DOI：
10.1007/s10709-008-9258-6
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发表时间：
2009-01-01
期刊：
影响因子：
1.5
通讯作者：
Mohan, Subburaman
中科院分区：
文献类型：
--
作者：
Yu, Hongrun;Edderkaoui, Bouchra;Mohan, Subburaman
通过鉴定调节骨密度的基因，将有助于骨质疏松症治疗策略的发展。一种方法是通过数量性状基因座（QTL）定位。然而，存在性别差异的存在/不存在和BMD QTL的位置。在以前的研究中，我们的小组确定了一个BMD QTL的17号染色体上的F-2雌性小鼠的MRL/MpJ x SJL/J的交叉。在这里，我们确定它是否也存在于同一杂交的雄性小鼠中。此外，我们还打算通过增加标记密度来减少QTL区域。区间作图显示，基于染色体位置的相同QTL存在于雄性小鼠中，股骨BMD的对数优势（LOD）评分为4.0，全身BMD为5.2。虽然在同一位置存在一个体重QTL，但BMD QTL不受体重调整的影响。增加标记密度的作图表明该QTL最可能的区域为35-55 Mb。股骨长、股骨骨膜周长和全身骨面积也存在共定位的QTL，提示可能存在多效性。Runx 2和VEGFA是位于该QTL区域内的强候选基因。
Developing treatment strategies for osteoporosis would be facilitated by identifying genes regulating bone mineral density (BMD). One way to do so is through quantitative trait locus (QTL) mapping. However, there are sex differences in terms of the presence/absence and locations of BMD QTLs. In a previous study, our group identified a BMD QTL on chromosome 17 in the F-2 female mice of the MRL/MpJ x SJL/J cross. Here, we determined whether it was also present in the male mice of the same cross. Furthermore, we also intended to reduce the QTL region by increasing marker density. Interval mapping showed that the same QTL based on chromosomal positions was present in the male mice, with logarithmic odds (LOD) scores of 4.0 for femur BMD and 5.2 for total body BMD. Although there was a body weight QTL at the same location, the BMD QTL was not affected by the adjustment for body weight. Mapping with increased marker density indicated a most likely region of 35-55 Mb for this QTL. There were also co-localized QTLs for femur length, femur periosteal circumference (PC) and total body bone area, suggesting possibility of pleiotropy. Runx2 and VEGFA are strong candidate genes located within this QTL region.